Human PATL1 (Pat1b) knockout HeLa cell lysate
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PATL1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 6 and Insertion of the selection cassette in exon 6.
View Alternative Names
OK/KNS-cl.5, PAT1-like protein 1, PATL1_HUMAN, Pat1b, Protein PAT1 homolog 1, Protein PAT1 homolog b, hPat1b, protein associated with topoisomerase II homolog 1
- Sanger seq
Unknown
Sanger Sequencing - Human PATL1 (Pat1b) knockout HeLa cell lysate (AB259033)
Allele-2 : Insertion of the selection cassette in exon 6
- Sanger seq
Unknown
Sanger Sequencing - Human PATL1 (Pat1b) knockout HeLa cell lysate (AB259033)
Allele-1 : 1 bp insertion in exon 6
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The degradation of mRNA represents a critical step where Pat1b plays an essential role. As a component of the Pat1-Lsm complex Pat1b interacts with several proteins to regulate the stability and translation of mRNA. Pat1b influences the mRNA decapping process which is vital for controlled mRNA degradation thereby controlling gene expression post-transcriptionally. This process ensures the removal of aberrant or unnecessary mRNAs from the cellular environment enabling the cell to adapt to new conditions or signals rapidly.
Pathways
MRNA decay and processing pathways involve Pat1b as a central component. Within the mRNA surveillance pathway Pat1b cooperates with Dcp1-Dcp2 decapping enzymes to mediate mRNA decay. Pat1b is pivotal in the nonsense-mediated mRNA decay (NMD) pathway where it partners with Upf1 to target and degrade mRNAs containing premature stop codons. These pathways ensure cellular integrity and protein synthesis regulation.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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