Human PBK (SPK) knockout HEK-293T cell lysate
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PBK KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 2.
View Alternative Names
CT84, Cancer/testis antigen 84, Epididymis luminal protein 164, FLJ14385, HEL164, Lymphokine-activated killer T-cell-originated protein kinase, MAPKK-like protein kinase, Nori-3, PBK, PDZ-binding kinase, SPK, Serine/threonine protein kinase, Spermatogenesis-related protein kinase, T-LAK cell-originated protein kinase, TOPK_HUMAN
- WB
Lab
Western blot - Human PBK (SPK) knockout HEK-293T cell lysate (AB257575)
Lane 1 : Wild-type HEK-293T cell lysate (20µg)
Lane 2 : PBK knockout HEK-293T cell lysate (20µg)
Lanes 1- 2 : Merged signal (red and green). Green - ab75987 observed at 40 kDa. Red - loading control ab7291 observed at 50 kDa.
ab75987 Anti-PBK/SPK antibody [EP2520Y] was shown to specifically react with PBK/SPK in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266827 (knockout cell lysate ab257575) was used. Wild-type and PBK/SPK knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab75987 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 100000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-PBK/SPK antibody [EP2520Y] (<a href='/en-us/products/primary-antibodies/pbk-spk-antibody-ep2520y-ab75987'>ab75987</a>) at 1/100000 dilution
Lane 1:
Wild-type HEK-293T cell lysate at 20 µg
Lane 2:
PBK knockout HEK-293T cell lysate at 20 µg
Lane 2:
Western blot - Human PBK (SPK) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-pbk-spk-knockout-hek-293t-cell-line-ab266827'>ab266827</a>)
Predicted band size: 27 kDa,36 kDa
Observed band size: 40 kDa
false
- WB
Lab
Western blot - Human PBK (SPK) knockout HEK-293T cell lysate (AB257575)
Lane 1 : Wild-type HEK-293T cell lysate (20µg)
Lane 2 : PBK knockout HEK-293T cell lysate (20µg)
Lanes 1- 2 : Merged signal (red and green). Green - ab236871 observed at 40 kDa. Red - loading control ab7291 observed at 50 kDa.
ab236871 Anti-PBK/SPK antibody [EPR21982] was shown to specifically react with PBK/SPK in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266827 (knockout cell lysate ab257575) was used. Wild-type and PBK/SPK knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab236871 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-PBK/SPK antibody [EPR21982] (<a href='/en-us/products/primary-antibodies/pbk-spk-antibody-epr21982-ab236871'>ab236871</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK-293T cell lysate at 20 µg
Lane 2:
PBK knockout HEK-293T cell lysate at 20 µg
Lane 2:
Western blot - Human PBK (SPK) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-pbk-spk-knockout-hek-293t-cell-line-ab266827'>ab266827</a>)
Predicted band size: 36 kDa
Observed band size: 40 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human PBK (SPK) knockout HEK-293T cell lysate (AB257575)
Homozygous : 1 bp deletion in exon 2
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PBK/SPK facilitates cell cycle progression and cellular proliferation. This protein is not typically part of larger complexes but it partners with other molecules during cell division to ensure proper mitotic spindle formation and chromosome segregation. Its expression levels increase during the G2/M phase of the cell cycle indicating its involvement in cell cycle regulation. In cancer it often shows overexpression contributing to unregulated proliferation of cancer cells.
Pathways
PBK/SPK is associated with critical cell division pathways such as the MAPK signaling pathway. This involvement impacts cellular responses to growth signals and stress. PBK can phosphorylate and interact with proteins like p38 MAPK which further modulates signal transduction related to cellular growth and survival. Through these pathways PBK/SPK helps manage the balance between cell division and apoptosis particularly under stress conditions.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PBK/SPK antibody [EPR21983] (AB236872)](https://content.abcam.com/products/images/pbk-spk-antibody-epr21983-ab236872--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img130999.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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