Human PCNP knockout HEK-293T cell lysate
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PCNP KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 13 bp deletion in exon1 and 1 bp insertion in exon1.
View Alternative Names
AI647035, Ab2 416, PCNP_HUMAN, PEST proteolytic signal-containing nuclear protein, PEST-containing nuclear protein
- Sanger seq
Unknown
Sanger Sequencing - Human PCNP knockout HEK-293T cell lysate (AB259034)
Allele-1 : 13 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human PCNP knockout HEK-293T cell lysate (AB259034)
Allele-2 : 1 bp insertion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This protein participates in cell cycle regulation and ubiquitination pathways suggesting its role in protein turnover within cells. PCNP often interacts with other proteins as part of larger complexes to influence proteolysis and cell cycle progression. The interaction with molecules containing ubiquitin-like domains indicates its involvement in tagging specific proteins for degradation helping maintain cellular homeostasis.
Pathways
PCNP connects strongly to the ubiquitin-proteasome pathway and the regulation of transcription. Its interactions with proteins such as RNF115 indicate its role in protein ubiquitination a fundamental process for maintaining cell integrity and function. Another relevant link is to cyclin-dependent kinases suggesting a contribution to cell cycle checkpoints and transitions essential for controlled cell division and proliferation.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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