PCOLCE2 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon1 and 16 bp deletion in exon1.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon1 and 16 bp deletion in exon1.
Alternative names
2400001O18Rik, MGC187469, PCOC2_HUMAN, PCOLCE 2, PCOLE 2, PCPE-2, Procollagen C-endopeptidase enhancer 2, Procollagen C-proteinase enhancer 2, Procollagen COOH-terminal proteinase enhancer 2
What's included?
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PCOLCE2 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon1 and 16 bp deletion in exon1.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon1 and 16 bp deletion in exon1.
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- PCOLCE2
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PCPE-2 also known as procollagen C-endopeptidase enhancer 2 enhances the activity of procollagen C-proteinase. It weighs approximately 42 kDa. PCPE-2 is expressed in various tissues including liver and kidney. This protein influences collagen biosynthesis by binding to substrates and increasing enzymatic activity.
- Biological function summary
PCPE-2 assists in the maturation of type I II and III procollagens. It does not form a part of any larger complex but it increases the cleavage efficiency of the C-terminal propeptide from procollagens. By promoting collagen fibril formation PCPE-2 plays a role in tissue organization and structure.
- Pathways
The role of PCPE-2 in collagen biosynthesis connects it to the extracellular matrix organization pathway. Additionally this protein interacts with enzymes such as bone morphogenetic protein 1 (BMP1) which are important for collagen maturation. These interactions facilitate the assembly and structure of the extracellular matrix.
- Associated diseases and disorders
PCPE-2's involvement in collagen maturation links it to fibrosis a condition characterized by excessive collagen deposition. It also relates to osteogenesis imperfecta a disorder affecting bone strength. In these conditions PCPE-2 works alongside proteins like BMP1 influencing collagen processing and accumulation thereby affecting tissue characteristics.
Product promise
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2 product images
Sanger Sequencing - Human PCOLCE2 (PCPE-2) knockout HeLa cell lysate (ab259035)
Allele-1: 16 bp deletion in exon1
Sanger Sequencing - Human PCOLCE2 (PCPE-2) knockout HeLa cell lysate (ab259035)
Allele-2: 14 bp deletion in exon1
Downloads
Product protocols
- Visit the general protocols
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