Human PDCD10 (CCM3) knockout HeLa cell lysate
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PDCD10 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon3 and Insertion of the selection cassette in exon3.
View Alternative Names
Apoptosis related protein 15, CCM3, Cerebral cavernous malformations 3 protein, MGC1212, MGC24477, PDC10_HUMAN, PDCD 10, Programmed cell death 10, Programmed cell death protein 10, TF-1 cell apoptosis-related protein 15, TFAR15
- Sanger seq
Unknown
Sanger Sequencing - Human PDCD10 (CCM3) knockout HeLa cell lysate (AB258096)
Allele-2 : Insertion of the selection cassette in exon3
- Sanger seq
Unknown
Sanger Sequencing - Human PDCD10 (CCM3) knockout HeLa cell lysate (AB258096)
Allele-1 : 1 bp insertion in exon3
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PDCD10 interacts with several proteins and complexes to perform its regulatory roles. It forms interactions with the GCKIII kinases which mediate its function in maintaining vascular stability and cell proliferation. Within these interactions PDCD10 helps to promote the structural integrity of endothelial cells contributing to the prevention of vascular leakage. This protein also plays a role in apoptosis regulation engaging with apoptotic pathways to guide cell death mechanisms.
Pathways
PDCD10 significantly contributes to the MAPK signaling pathway and the PI3K/AKT pathway. These pathways are vital for regulating cell growth survival and differentiation. PDCD10 acts alongside proteins such as KRIT1 and PDCD10-L to mediate cellular signaling cascades modulating how cells respond to internal and external stimuli. Through these pathways PDCD10 influences cellular outcomes that are critical for proper organ development and function.
Quality control
STR analysis
D5S818, TH01, D16S539, TPOX, CSF1PO, D13S317, D7S820
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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