PDCD10 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon3 and Insertion of the selection cassette in exon3.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon3 and Insertion of the selection cassette in exon3.
Alternative names
Apoptosis related protein 15, CCM3, Cerebral cavernous malformations 3 protein, MGC1212, MGC24477, PDC10_HUMAN, PDCD 10, Programmed cell death 10, Programmed cell death protein 10, TF-1 cell apoptosis-related protein 15, TFAR15
What's included?
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PDCD10 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon3 and Insertion of the selection cassette in exon3.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon3 and Insertion of the selection cassette in exon3.
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- PDCD10
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- D5S818, TH01, D16S539, TPOX, CSF1PO, D13S317, D7S820
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The PDCD10 protein also known as CCM3 is an important regulatory protein with an important role in cellular processes. It has a molecular mass of around 23 kDa and participates in various cellular mechanisms. PDCD10 expresses highly in the brain liver heart and kidneys which indicates its broad impact on different organ systems. The intricate nature of PDCD10’s function aligns with its presence in essential cellular environments.
- Biological function summary
PDCD10 interacts with several proteins and complexes to perform its regulatory roles. It forms interactions with the GCKIII kinases which mediate its function in maintaining vascular stability and cell proliferation. Within these interactions PDCD10 helps to promote the structural integrity of endothelial cells contributing to the prevention of vascular leakage. This protein also plays a role in apoptosis regulation engaging with apoptotic pathways to guide cell death mechanisms.
- Pathways
PDCD10 significantly contributes to the MAPK signaling pathway and the PI3K/AKT pathway. These pathways are vital for regulating cell growth survival and differentiation. PDCD10 acts alongside proteins such as KRIT1 and PDCD10-L to mediate cellular signaling cascades modulating how cells respond to internal and external stimuli. Through these pathways PDCD10 influences cellular outcomes that are critical for proper organ development and function.
- Associated diseases and disorders
PDCD10 holds a strong association with cerebral cavernous malformations (CCMs) a condition involving abnormal blood vessels in the brain. Mutations in the PDCD10 gene lead to dysfunctional protein products contributing to the pathogenesis of CCMs. This condition connects PDCD10 with other proteins like KRIT1 and CCM2 which also contribute to CCM pathology. Additionally PDCD10 has implications in cancer biology due to its involvement in regulating apoptosis and cell proliferation linking it to oncogenic processes when dysregulated.
Product promise
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Terms & Conditions.
2 product images
Sanger Sequencing - Human PDCD10 (CCM3) knockout HeLa cell lysate (ab258096)
Allele-1: 1 bp insertion in exon3
Sanger Sequencing - Human PDCD10 (CCM3) knockout HeLa cell lysate (ab258096)
Allele-2: Insertion of the selection cassette in exon3
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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