Human PDCD6 (ALG-2) knockout HEK-293T cell lysate
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PDCD6 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 10 bp deletion in exon 1.
View Alternative Names
ALG-2-interacting protein 1, Apoptosis linked gene 2, Apoptosis-linked gene 2 protein, FLJ42309, FLJ46208, Hp95, KIAA1375, MA 3, MGC111017, MGC119050, MGC9123, PDCD6_HUMAN, PEF 1B, PS-2, Probable calcium-binding protein ALG-2, Programmed cell death 6, Programmed cell death 6-interacting protein, Programmed cell death protein 6
- WB
Lab
Western blot - Human PDCD6 (ALG-2) knockout HEK-293T cell lysate (AB258570)
Lane 1 : Wild-type HEK293T cell lysate (20 ug)
Lane 2 : PDCD6 knockout HEK293T cell lysate (20 ug)
Lane 3 : HeLa cell lysate (20 ug)
Lane 4 : Daudi cell lysate (20 ug)
ab109181 was shown to specifically react with PDCD6/ALG-2 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266323 (knockout cell lysate ab258570) was used. Wild-type and PDCD6/ALG-2 knockout samples were subjected to SDS-PAGE. ab109181 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-PDCD6/ALG-2 antibody [EP3259] (<a href='/en-us/products/primary-antibodies/pdcd6-alg-2-antibody-ep3259-ab109181'>ab109181</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK293T cell lysate at 20 µg
Lane 2:
PDCD6 knockout HEK293T cell lysate at 20 µg
Lane 2:
Western blot - Human PDCD6 (ALG-2) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-pdcd6-alg-2-knockout-hek-293t-cell-line-ab266323'>ab266323</a>)
Lane 3:
HeLa cell lysate at 20 µg
Lane 4:
Daudi cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution
Predicted band size: 21 kDa
Observed band size: 22 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human PDCD6 (ALG-2) knockout HEK-293T cell lysate (AB258570)
Homozygous : 10 bp deletion in exon 1
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The function of PDCD6 involves its participation in calcium signaling pathways. This protein is part of a larger complex involving T-cell receptor signaling and also influences the Fas ligand-mediated apoptosis pathway. PDCD6 achieves its regulatory roles through interactions with various proteins such as MA3Ps. As a calcium-binding protein ALG-2 modulates calcium homeostasis which is critical for maintaining cellular functions including cell proliferation differentiation and programmed cell death.
Pathways
The involvement of PDCD6 in apoptosis is significant. It works in coordination with pathway proteins such as caspases and fas-associated proteins influencing cell death signaling. In addition PDCD6's ability to bind calcium allows it to fit into the broader intracellular signaling network placing it in connection with MAPK pathways essential for cellular responses to stress and growth signals. ALG protein interactions underline PDCD6's influence across apoptosis and signaling pathways.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
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Product promise
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