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AB275522

Human PDGFRA knockout SH-SY5Y cell lysate

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PDGFRA KO cell lysate available now. KO validated by Immunocytochemistry, Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 67 bp deletion in exon 3.

View Alternative Names

Alpha-type platelet-derived growth factor receptor, CD140 antigen-like family member A, CD140A, CD140a antigen, MGC74795, PDGF Receptor alpha, PDGF alpha chain, PDGF-R-alpha, PDGFR 2, PDGFRA, PDGFRA/BCR fusion, PGFRA_HUMAN, Platelet derived growth factor receptor, Platelet derived growth factor receptor 2, Platelet derived growth factor receptor alpha, Platelet derived growth factor receptor alpha polypeptide, RHEPDGFRA, Rearranged in hypereosinophilia platelet derived growth factor receptor alpha fusion protein

3 Images
Western blot - Human PDGFRA knockout SH-SY5Y cell lysate (AB275522)
  • WB

Lab

Western blot - Human PDGFRA knockout SH-SY5Y cell lysate (AB275522)

Lane 1 : Wild-type SH-SY5Y cell lysate 40 μg
Lane 2 : PDGFRA knockout SH-SY5Y cell lysate 40 μg
Lanes 1 - 2 : Merged signal (red and green). Green - ab203491 observed at 150 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab203491 was shown to react with PDGFR alpha in wild-type SH-SY5Y cells in Western blot with loss of signal observed in PDGFRA knockout cell line ab275335 (knockout cell lysate ab275522). Wild-type SH-SY5Y and PDGFRA knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab203491 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-PDGFR alpha antibody [EPR22059-270] (<a href='/en-us/products/primary-antibodies/pdgfr-alpha-antibody-epr22059-270-ab203491'>ab203491</a>) at 1/1000 dilution

Lane 1:

Wild-type SH-SY5Y cell lysate at 40 µg

Lane 2:

PDGFRA knockout SH-SY5Y cell lysate at 40 µg

Lane 2:

Western blot - Human PDGFRA knockout SH-SY5Y cell line (<a href='/en-us/products/cell-lines/human-pdgfra-knockout-sh-sy5y-cell-line-ab275335'>ab275335</a>)

Predicted band size: 122 kDa

Observed band size: 150 kDa

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Western blot - Human PDGFRA knockout SH-SY5Y cell lysate (AB275522)
  • WB

Lab

Western blot - Human PDGFRA knockout SH-SY5Y cell lysate (AB275522)

Lane 1 : Wild-type SH-SY5Y cell lysate 40 μg
Lane 2 : PDGFRA knockout SH-SY5Y cell lysate 40 μg
Lanes 1 - 2 : Merged signal (red and green). Green - ab134123 observed at 180 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab134123 was shown to react with PDGFR alpha in wild-type SH-SY5Y cells in Western blot with loss of signal observed in PDGFRA knockout cell line ab275335 (knockout cell lysate ab275522). Wild-type SH-SY5Y and PDGFRA knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab134123 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-PDGFR alpha antibody [EPR5480] (<a href='/en-us/products/primary-antibodies/pdgfr-alpha-antibody-epr5480-ab134123'>ab134123</a>) at 1/1000 dilution

Lane 1:

Wild-type SH-SY5Y cell lysate at 40 µg

Lane 2:

PDGFRA knockout SH-SY5Y cell lysate at 40 µg

Lane 2:

Western blot - Human PDGFRA knockout SH-SY5Y cell line (<a href='/en-us/products/cell-lines/human-pdgfra-knockout-sh-sy5y-cell-line-ab275335'>ab275335</a>)

Predicted band size: 122 kDa

Observed band size: 180 kDa

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Sanger Sequencing - Human PDGFRA knockout SH-SY5Y cell lysate (AB275522)
  • Sanger seq

Lab

Sanger Sequencing - Human PDGFRA knockout SH-SY5Y cell lysate (AB275522)

Allele-1 : 67 bp deletion in exon 3

Key facts

Cell type

SH-SY5Y

Species or organism

Human

Tissue

Bone marrow

Knockout validation

Immunocytochemistry,Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 67 bp deletion in exon 3

Disease

Neuroblastoma

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Reactivity data

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
PDGFRA
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Immunocytochemistry, Sanger Sequencing, Western blot
Zygosity
Homozygous
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDGFR alpha also known as platelet-derived growth factor receptor alpha is a cell surface receptor tyrosine kinase with a mass of approximately 123 kDa. It plays a mechanical role in transmitting signals across the cell membrane initiating cellular responses. PDGFR alpha is widely expressed in mesenchymal cells including fibroblasts and smooth muscle cells. Researchers frequently employ techniques such as PDGFRA IHC and PDGFRA staining to study the localization and expression levels in tissues.
Biological function summary

PDGFR alpha mediates cell proliferation survival and differentiation especially in embryonic development and wound healing. The receptor often forms a complex by dimerizing with itself or with PDGFR beta to activate intracellular signaling cascades. This process is initiated upon ligand binding which triggers autophosphorylation on tyrosine residues. The receptor is also recognized in the detection of PDGFRA with assays such as PDGFRA ELISA.

Pathways

PDGFR alpha significantly affects the MAPK and PI3K/AKT signaling pathways which regulate cellular growth and survival signals. Through these pathways PDGFR alpha interacts with related proteins like SHP-2 and PI3K contributing to signal transduction involved in cellular responses. This role links PDGFR alpha to important cellular processes via its kinase activity and signal integration.

PDGFR alpha is implicated in conditions such as gastrointestinal stromal tumors (GISTs) and idiopathic pulmonary fibrosis (IPF). It connects to proteins such as KIT in GISTs where mutations cause aberrant receptor signaling leading to tumorigenesis. Aberrant PDGFR alpha signaling also associates with IPF a disorder characterized by increased fibroblast proliferation and fibrotic tissue formation. These connections make PDGFR alpha a significant target for research and therapeutic intervention.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PDGFR alpha antibody [EPR22059-270] (AB203491)

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Cell Lines & Lysates

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Assay Kits

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Sandwich ELISA - Human PDGF R alpha ELISA Kit (AB242246)

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Primary Antibodies

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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