JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB275523

Human PDGFRB knockout SH-SY5Y cell lysate

Be the first to review this product! Submit a review

|

(0 Publication)

PDGFRB KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 5 bp deletion in exon 3.

View Alternative Names

Beta platelet derived growth factor receptor, Beta-type platelet-derived growth factor receptor, CD 140B, CD140 antigen-like family member B, CD140b antigen, IBGC4, IMF1, JTK12, OTTHUMP00000160528, PDGF Receptor beta, PDGF-R-beta, PDGFR, PDGFR 1, PDGFRB, PGFRB_HUMAN, Platelet derived growth factor receptor 1, Platelet derived growth factor receptor beta, Platelet derived growth factor receptor beta polypeptide

4 Images
Western blot - Human PDGFRB knockout SH-SY5Y cell lysate (AB275523)
  • WB

Lab

Western blot - Human PDGFRB knockout SH-SY5Y cell lysate (AB275523)

Lane 1 : Wild-type SH-SY5Y cell lysate 30 ug
Lane 2 : PDGFRB knockout SH-SY5Y cell lysate 30 ug
Lane 3 : Human Skeletal Muscle tissue lysate 30 ug
Lane 4 : HeLa cell lysate 30 ug
Lanes 1 - 4 : Merged signal (red and green). Green - ab69506 observed at 170 kDa. Red - loading control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55kDa.
ab69506 was shown to react with PDGFR beta in wild-type SH-SY5Y cells in Western blot with loss of signal observed in PDGFRB knockout cell line ab273749 (knockout cell lysate ab275523). Wild-type SH-SY5Y and PDGFRB knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab69506 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-PDGFR beta antibody [42G12] (<a href='/en-us/products/primary-antibodies/pdgfr-beta-antibody-42g12-ab69506'>ab69506</a>) at 1/1000 dilution

Lane 1:

Wild-type SH-SY5Y cell lysate at 30 µg

Lane 2:

PDGFRB knockout SH-SY5Y cell lysate at 30 µg

Lane 2:

Western blot - Human PDGFRB knockout SH-SY5Y cell line (<a href='/en-us/products/cell-lines/human-pdgfrb-knockout-sh-sy5y-cell-line-ab273749'>ab273749</a>)

Lane 3:

Human Skeletal Muscle tissue lysate at 30 µg

Lane 4:

HeLa cell lysate at 30 µg

Predicted band size: 123 kDa

Observed band size: 170 kDa

false

Western blot - Human PDGFRB knockout SH-SY5Y cell lysate (AB275523)
  • WB

Lab

Western blot - Human PDGFRB knockout SH-SY5Y cell lysate (AB275523)

Lane 1 : Wild-type SH-SY5Y cell lysate 30 ug
Lane 2 : PDGFRB knockout SH-SY5Y cell lysate 30 ug
Lane 3 : Human Skeletal Muscle tissue lysate 30 ug
Lane 4 : HeLa cell lysate 30 ug
Lanes 1 - 4 : Merged signal (red and green). Green - ab32570 observed at 170 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.
ab32570 was shown to react with PDGFRB in wild-type SH-SY5Y cells in Western blot with loss of signal observed in PDGFRB knockout cell line ab273749 (knockout cell lysate ab275523). Wild-type SH-SY5Y and PDGFRB knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab32570 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 5000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal (<a href='/en-us/products/primary-antibodies/pdgfr-alpha-pdgfr-beta-antibody-y92-c-terminal-ab32570'>ab32570</a>) at 1/5000 dilution

Lane 1:

Wild-type SH-SY5Y cell lysate at 30 µg

Lane 2:

PDGFR beta knockout SH-SY5Y cell lysate at 30 µg

Lane 2:

Western blot - Human PDGFRB knockout SH-SY5Y cell line (<a href='/en-us/products/cell-lines/human-pdgfrb-knockout-sh-sy5y-cell-line-ab273749'>ab273749</a>)

Lane 3:

Human Skeletal Muscle tissue lysate at 30 µg

Lane 4:

HeLa cell lysate at 30 µg

Predicted band size: 123 kDa

Observed band size: 170 kDa

false

Sanger Sequencing - Human PDGFRB knockout SH-SY5Y cell lysate (AB275523)
  • Sanger seq

Lab

Sanger Sequencing - Human PDGFRB knockout SH-SY5Y cell lysate (AB275523)

Allele-1 : 5 bp deletion in exon 3

Western blot - Human PDGFRB knockout SH-SY5Y cell lysate (AB275523)
  • WB

Supplier Data

Western blot - Human PDGFRB knockout SH-SY5Y cell lysate (AB275523)

All lanes:

Western blot - Anti-PDGFR beta antibody [EPR26830-84] (<a href='/en-us/products/primary-antibodies/pdgfr-beta-antibody-epr26830-84-ab313777'>ab313777</a>) at 1/1000 dilution

Lane 1:

Wild-type SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

Western blot - Human PDGFRB knockout SH-SY5Y cell lysate (ab275523) at 20 µg

Lane 3:

MG-63 (human osteosarcoma fibroblast) whole cell lysate at 20 µg

Lane 4:

U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 200 kDa,150 kDa

true

Exposure time: 180s

Key facts

Cell type

SH-SY5Y

Species or organism

Human

Tissue

Bone marrow

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 5 bp deletion in exon 3

Disease

Neuroblastoma

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

style
left-column

What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab275523-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human PDGFRB knockout SHSY-5Y cell lysate", "number":"AB275523-CMP02", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type SHSY-5Y cell lysate", "number":"AB275523-CMP01", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Gene name
PDGFRB
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Zygosity
Homozygous
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-80°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PDGFR beta also known as PDGFR-b or PDGFRB is a cell surface tyrosine kinase receptor with a molecular mass of about 180 kDa. It binds the PDGF (platelet-derived growth factor) family of ligands. PDGFR beta is commonly found in various tissues including blood vessels and connective tissues and is highly expressed in cells like pericytes and fibroblasts. The receptor plays a critical role in cell signaling mechanisms involving proliferation chemotaxis and survival.
Biological function summary

The receptor plays an essential role in the regulation of cell growth and development. PDGFR beta undergoes dimerization and autophosphorylation upon ligand binding initiating a series of downstream signaling cascades. This receptor is often part of a complex with other receptor proteins promoting interactions necessary for signal propagation. Its main biological functions include mediating cellular responses to environmental signals that contribute to tissue repair and angiogenesis.

Pathways

PDGFR beta is an important player within the PI3K-Akt and MAPK signaling pathways. It works alongside proteins such as PI3K and Ras to regulate cellular responses related to growth and survival. These pathways facilitate cross-talk with other cellular processes influencing various cellular outcomes. This receptor's activity regulates critical physiological functions by providing signals that maintain cellular homeostasis under various physiological conditions.

PDGFR beta has significant implications in the progression of cancer and fibrotic diseases. Its overexpression or mutation can lead to anomalous signaling that contributes to tumorigenesis particularly in connective tissue tumors known as sarcomas. Additionally PDGFR beta's role in promoting fibroblast activity makes it relevant in fibrotic diseases such as pulmonary fibrosis. Abnormal activation of PDGFR beta can interact with related proteins like VEGF receptors enhancing pathogenic responses and contributing to disease severity.
style
left-column

Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

style
left-column

Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Female

style
left-column

Product protocols

style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB32570

Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Western blot - Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal (AB32570)

  • 4
  • 31
Assay Kits

AB253225

Mouse PDGFR beta ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Mouse PDGFR beta ELISA Kit (AB253225)

  • 0
  • 0
Proteins & Peptides

AB60833

Recombinant human PDGFR beta protein

Functional Studies - Recombinant human PDGFR beta protein (AB60833)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com