PDGFRB KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 5 bp deletion in exon 3.
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Key facts
- Cell type
SH-SY5Y
- Species or organism
Human
- Tissue
Bone marrow
- Knockout validation
Sanger Sequencing, Western blot
- Mutation description
Knockout achieved by using CRISPR/Cas9, Homozygous: 5 bp deletion in exon 3
Alternative names
Beta platelet derived growth factor receptor, Beta-type platelet-derived growth factor receptor, CD 140B, CD140 antigen-like family member B, CD140b antigen, IBGC4, IMF1, JTK12, OTTHUMP00000160528, PDGF Receptor beta, PDGF-R-beta, PDGFR, PDGFR 1, PDGFRB, PGFRB_HUMAN, Platelet derived growth factor receptor 1, Platelet derived growth factor receptor beta, Platelet derived growth factor receptor beta polypeptide
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PDGFRB KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 5 bp deletion in exon 3.
Key facts
- Cell type
SH-SY5Y
- Species or organism
Human
- Tissue
Bone marrow
- Knockout validation
Sanger Sequencing, Western blot
- Mutation description
Knockout achieved by using CRISPR/Cas9, Homozygous: 5 bp deletion in exon 3
- Disease
Neuroblastoma
- Concentration
Properties
- Gene name
PDGFRB
- Gene editing type
Knockout
- Gene editing method
CRISPR technology
- Knockout validation
Sanger Sequencing, Western blot
- Zygosity
Homozygous
Quality control
- STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
EU: 1 US: 1
- Adherent/suspension
Adherent
- Gender
Female
Storage
- Shipped at conditions
Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
-80°C
- Appropriate long-term storage conditions
-20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PDGFR beta also known as PDGFR-b or PDGFRB is a cell surface tyrosine kinase receptor with a molecular mass of about 180 kDa. It binds the PDGF (platelet-derived growth factor) family of ligands. PDGFR beta is commonly found in various tissues including blood vessels and connective tissues and is highly expressed in cells like pericytes and fibroblasts. The receptor plays a critical role in cell signaling mechanisms involving proliferation chemotaxis and survival.
- Biological function summary
The receptor plays an essential role in the regulation of cell growth and development. PDGFR beta undergoes dimerization and autophosphorylation upon ligand binding initiating a series of downstream signaling cascades. This receptor is often part of a complex with other receptor proteins promoting interactions necessary for signal propagation. Its main biological functions include mediating cellular responses to environmental signals that contribute to tissue repair and angiogenesis.
- Pathways
PDGFR beta is an important player within the PI3K-Akt and MAPK signaling pathways. It works alongside proteins such as PI3K and Ras to regulate cellular responses related to growth and survival. These pathways facilitate cross-talk with other cellular processes influencing various cellular outcomes. This receptor's activity regulates critical physiological functions by providing signals that maintain cellular homeostasis under various physiological conditions.
- Associated diseases and disorders
PDGFR beta has significant implications in the progression of cancer and fibrotic diseases. Its overexpression or mutation can lead to anomalous signaling that contributes to tumorigenesis particularly in connective tissue tumors known as sarcomas. Additionally PDGFR beta's role in promoting fibroblast activity makes it relevant in fibrotic diseases such as pulmonary fibrosis. Abnormal activation of PDGFR beta can interact with related proteins like VEGF receptors enhancing pathogenic responses and contributing to disease severity.
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4 product images
Western blot - Human PDGFRB knockout SH-SY5Y cell lysate (ab275523)
Lane 1: Wild-type SH-SY5Y cell lysate 30 ug
Lane 2: PDGFRB knockout SH-SY5Y cell lysate 30 ug
Lane 3: Human Skeletal Muscle tissue lysate 30 ug
Lane 4: HeLa cell lysate 30 ug
Lanes 1 - 4:Merged signal (red and green). Green - Anti-PDGFR beta antibody [42G12] ab69506 observed at 170 kDa. Red - loading control Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55kDa.
Anti-PDGFR beta antibody [42G12] ab69506 was shown to react with PDGFR beta in wild-type SH-SY5Y cells in Western blot with loss of signal observed in PDGFRB knockout cell line Human PDGFRB knockout SH-SY5Y cell line ab273749 (knockout cell lysate ab275523). Wild-type SH-SY5Y and PDGFRB knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with Anti-PDGFR beta antibody [42G12] ab69506 and Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.All lanes: Western blot - Anti-PDGFR beta antibody [42G12] (Anti-PDGFR beta antibody [42G12] ab69506) at 1/1000 dilution
Lane 1: Wild-type SH-SY5Y cell lysate at 30 µg
Lane 2: PDGFRB knockout SH-SY5Y cell lysate at 30 µg
Lane 3: Human Skeletal Muscle tissue lysate at 30 µg
Lane 4: HeLa cell lysate at 30 µg
Performed under reducing conditions.
Predicted band size: 123 kDa
Observed band size: 170 kDa
Western blot - Human PDGFRB knockout SH-SY5Y cell lysate (ab275523)
Lane 1: Wild-type SH-SY5Y cell lysate 30 ug
Lane 2: PDGFRB knockout SH-SY5Y cell lysate 30 ug
Lane 3: Human Skeletal Muscle tissue lysate 30 ug
Lane 4: HeLa cell lysate 30 ug
Lanes 1 - 4:Merged signal (red and green). Green - Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal ab32570 observed at 170 kDa. Red - loading control Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.
Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal ab32570 was shown to react with PDGFRB in wild-type SH-SY5Y cells in Western blot with loss of signal observed in PDGFRB knockout cell line Human PDGFRB knockout SH-SY5Y cell line ab273749 (knockout cell lysate ab275523). Wild-type SH-SY5Y and PDGFRB knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal ab32570 and Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 5000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.All lanes: Western blot - Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal (Anti-PDGFR alpha + PDGFR beta antibody [Y92] - C-terminal ab32570) at 1/5000 dilution
Lane 1: Wild-type SH-SY5Y cell lysate at 30 µg
Lane 2: PDGFR beta knockout SH-SY5Y cell lysate at 30 µg
Lane 3: Human Skeletal Muscle tissue lysate at 30 µg
Lane 4: HeLa cell lysate at 30 µg
Performed under reducing conditions.
Predicted band size: 123 kDa
Observed band size: 170 kDa
Western blot - Human PDGFRB knockout SH-SY5Y cell lysate (ab275523)
All lanes: Western blot - Anti-PDGFR beta antibody [EPR26830-84] (Anti-PDGFR beta antibody [EPR26830-84] ab313777) at 1/1000 dilution
Lane 1: Wild-type SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg
Lane 2: Western blot - Human PDGFRB knockout SH-SY5Y cell lysate (ab275523) at 20 µg
Lane 3: MG-63 (human osteosarcoma fibroblast) whole cell lysate at 20 µg
Lane 4: U-87 MG (human glioblastoma-astrocytoma epithelial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 200 kDa, 150 kDa
Exposure time: 180s
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 28334876, 19246520, 11346654).
In Western blot, Anti-PDGFR beta antibody [EPR26830-84] ab313777 was shown to bind specifically to PDGFR beta. Two bands were observed at 200 kDa and 150 kDa in wild-type SH-SY5Y cell lysates ( (lane 1) whereas no signal was observed at this size in PDGFRB knockout cell line (lane 2, knockout cell line Human PDGFRB knockout SH-SY5Y cell line ab273749/ knockout cell lysate ab275523).
This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.
In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
Sanger Sequencing - Human PDGFRB knockout SH-SY5Y cell lysate (ab275523)
Allele-1: 5 bp deletion in exon 3
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