Human PDIA5 knockout HeLa cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human PDIA5 knockout HeLa cell lysate (AB263293)
Homozygous : 7 bp deletion in exon2
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PDIA5 has important roles in regulating redox homeostasis and protein quality control within the endoplasmic reticulum. It does not operate alone; it functions as part of multi-protein complexes engaging with other chaperones to assist in folding newly synthesized proteins. These interactions prevent protein aggregation and accumulation of misfolded proteins protecting cells from stress.
Pathways
PDIA5 integrates into the unfolded protein response (UPR) and endoplasmic reticulum-associated degradation (ERAD) pathways. It interfaces with other proteins like BiP/GRP78 which enhances its chaperone activity during cellular stress and EDEM1 a protein involved in recognizing misfolded glycoproteins. These pathways work together to maintain cellular homeostasis by managing protein load and degradation processes.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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