PDXK KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and Insertion of the selection cassette in exon1.
Images
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and Insertion of the selection cassette in exon1.
Alternative names
PDXK_HUMAN, PKH, PNK, Pyridoxal kinase, Pyridoxamine kinase, Pyridoxine kinase, Pyridoxine kinase;, Vitamin B6 kinase
What's included?
Recommended products
PDXK KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and Insertion of the selection cassette in exon1.
Key facts
- Cell type
- HEK-293T
- Species or organism
- Human
- Tissue
- Kidney
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and Insertion of the selection cassette in exon1.
- Concentration
Properties
- Gene name
- PDXK
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PDXK.1 also known as pyridoxal kinase is an important enzyme that facilitates the phosphorylation of vitamin B6 to pyridoxal-5'-phosphate (PLP). Its molecular mass is approximately 35 kDa. You can find it expressed mainly in the liver kidney and brain tissues. PDXK.1 plays a role in converting vitamin B6 which is necessary for various enzymatic activities throughout the body.
- Biological function summary
PDXK.1 functions within cellular environments by catalyzing the formation of PLP an active form of vitamin B6. PLP acts as a coenzyme involved in numerous reactions such as amino acid metabolism neurotransmitter synthesis and modulation of hormone activity. PDXK.1 does not work in isolation but instead interacts with other proteins in these processes though it is not a part of a larger complex.
- Pathways
PDXK.1 is important for the vitamin B6 metabolism pathway. This pathway integrates with networks involving amino acid catabolism and neurotransmitter pathways essential for metabolic functions and nervous system regulation. An association exists between PDXK.1 and proteins like cysteine desulfurase which also rely on PLP for their enzymatic activities highlighting its involvement in broader biochemical systems.
- Associated diseases and disorders
PDXK.1 has been linked with metabolic disorders and neurological conditions. A deficiency or dysfunction in PDXK.1 activity can contribute to conditions such as pyridoxine-dependent epilepsy and some forms of anemia. In these cases disruptions in the metabolism of vitamin B6 due to inadequate levels of PLP may directly affect proteins like glutamate decarboxylase which require PLP for proper function.
Product promise
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Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Sanger Sequencing - Human PDXK (PDXK.1) knockout HEK-293T cell lysate (ab259036)
Allele-1: 1 bp insertion in exon1
Sanger Sequencing - Human PDXK (PDXK.1) knockout HEK-293T cell lysate (ab259036)
Allele-2: Insertion of the selection cassette in exon1
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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