Human PEG10 (EDR) knockout HeLa cell lysate
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PEG10 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2.
View Alternative Names
AA407948, Edr, Embryonal carcinoma differentiation regulated, Embryonal carcinoma differentiation-regulated protein, HB 1, KIAA1051, MEF3 like 1, MEF3-like protein 1, MEF3L, MEF3L1, Mammalian retrotransposon-derived protein 2, Mar2, Mart2, MyEF 3, Myelin expression factor 3-like protein 1, PEG10 protein, PEG10_HUMAN, Paternally expressed 10, Paternally expressed gene 10 ORF1, Paternally expressed gene 10 protein, Putative uncharacterized protein PEG10, RGAG3, Retrotransposon gag domain containing 3, Retrotransposon gag domain-containing protein 3, Retrotransposon-derived gag-like polyprotein, Retrotransposon-derived protein PEG10, Ty3/Gypsy-like protein
- WB
Lab
Western blot - Human PEG10 (EDR) knockout HeLa cell lysate (AB258103)
Lane 1 : Wild-type HeLa cell lysate (20 μg)
Lane 2 : PEG10 knockout HeLa cell lysate (20 μg)
Lane 3 : HepG2 cell lysate (20 μg)
Lanes 1-4 : Merged signal (red and green). Green - ab215035 observed at 100 kDa. Red - loading control ab7291 observed at 50 kDa.
ab215035 Anti-PEG10/EDR antibody [EPR20051] was shown to specifically react with PEG10/EDR in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265925 (knockout cell lysate ab258103) was used. Wild-type and PEG10/EDR knockout samples were subjected to SDS-PAGE. ab215035 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-PEG10/EDR antibody [EPR20051] (<a href='/en-us/products/primary-antibodies/peg10-edr-antibody-epr20051-ab215035'>ab215035</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
PEG10 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human PEG10 (EDR) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-peg10-edr-knockout-hela-cell-line-ab265925'>ab265925</a>)
Lane 3:
HepG2 cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution
Predicted band size: 80 kDa
Observed band size: 100 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human PEG10 (EDR) knockout HeLa cell lysate (AB258103)
Homozygous : 1 bp deletion in exon2
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The PEG10 protein plays multiple roles especially in cell proliferation apoptosis inhibition and promoting cell survival. It can be part of larger protein complexes that modulate cellular responses to stress and developmental cues. The protein's multifaceted activities are important during embryogenesis where it supports trophoblast development and placental formation.
Pathways
PEG10 interacts with signaling cascades such as the TGF-beta signaling pathway and is involved in cell cycle regulation. PEG10 works alongside proteins like paternally expressed genes that connect with TGF-beta pathways influencing cellular differentiation and growth. Its integration in these pathways highlights its role in managing cellular environments and responses to cellular stress signals.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB215035
Anti-PEG10/EDR antibody [EPR20051]
RabMAb|Recombinant|KO Validated|20ul selling size|Trial Size|Trial Size
![Immunocytochemistry/ Immunofluorescence - Anti-PEG10/EDR antibody [EPR20051] (AB215035)](https://content.abcam.com/products/images/peg10-edr-antibody-epr20051-ab215035--immunocytochemistry-immunofluorescence-img108418.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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