JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB258105

Human PERP knockout HeLa cell lysate

Be the first to review this product! Submit a review

|

(0 Publication)

PERP KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 70 bp insertion in exon1 and Insertion of the selection cassette in exon1.
3 Images
Western blot - Human PERP knockout HeLa cell lysate (AB258105)
  • WB

Lab

Western blot - Human PERP knockout HeLa cell lysate (AB258105)

Lane 1 : Wild-type HeLa cell lysate (20 ug)
Lane 2 : PERP knockout HeLa cell lysate (20 ug)
Lane 3 : HaCaT cell lysate (20 ug)

ab129083 was shown to specifically react with PERP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265335 (knockout cell lysate ab258105) was used. Wild-type and PERP knockout samples were subjected to SDS-PAGE. ab129083 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PERP antibody [EPR7885] (<a href='/en-us/products/primary-antibodies/perp-antibody-epr7885-ab129083'>ab129083</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

PERP knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human PERP knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-perp-knockout-hela-cell-line-ab265335'>ab265335</a>)

Lane 3:

HaCaT cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 21 kDa

Observed band size: 24 kDa

false

Sanger Sequencing - Human PERP knockout HeLa cell lysate (AB258105)
  • Sanger seq

Unknown

Sanger Sequencing - Human PERP knockout HeLa cell lysate (AB258105)

Allele-1 : Insertion of the selection cassette in exon1

Sanger Sequencing - Human PERP knockout HeLa cell lysate (AB258105)
  • Sanger seq

Unknown

Sanger Sequencing - Human PERP knockout HeLa cell lysate (AB258105)

Allele-2 : 70 bp insertion in exon1

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 70 bp insertion in exon1 and Insertion of the selection cassette in exon1.

Disease

Adenocarcinoma

style
left-column

Complementary Products

Proteins & Peptides

AB259412

Recombinant human VEGF 165A protein (Active)

Premium Bioactive

Mass Spectrometry - Recombinant human VEGF 165A protein (Active) (AB259412)

  • 0
  • 0
Primary Antibodies

AB129083

Anti-PERP antibody [EPR7885]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-PERP antibody [EPR7885] (AB129083)

  • 0
  • 0
Proteins & Peptides

AB288799

Recombinant Human VCAM1 protein (Active)

Premium Bioactive

Biological Activity - Recombinant Human VCAM1 protein (Active) (AB288799)

  • 0
  • 0
Proteins & Peptides

AB52101

Recombinant human Cleaved Caspase-3 protein (Active)

Western blot - Recombinant human Cleaved Caspase-3 protein (Active) (AB52101)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

style
left-column

What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab258105-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human PERP knockout HeLa cell lysate", "number":"AB258105-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HeLa cell lysate", "number":"AB258105-CMP02", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Gene name
PERP
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PERP also known as P53 apoptosis effector related to PMP-22 is a protein with an approximate mass of 23 kDa. It mainly operates as an effector in the p53-dependent apoptosis pathway. PERP is known for facilitating cell-cell adhesion as a part of the desmosome complex. Researchers have found its expression primarily in epithelial tissues where it contributes to maintaining cell structure integrity. PERP's role is significant in tissues experiencing stress where it upregulates in response to p53 activation.
Biological function summary

PERP plays an active role in cellular processes like adhesion and apoptosis. As a part of the desmosome it helps in connecting epithelial cells. This function is important for preserving tissue architecture and responding to mechanical stress. PERP's involvement in apoptosis arises from its interactions in the p53 pathway supporting the process when cells require programmed death. These roles are essential for maintaining balanced cell proliferation and death key for tissue homeostasis.

Pathways

PERP is closely tied to the p53 pathway and the desmosomal adhesion process. The p53 pathway involves one of the primary tumor suppressor proteins and regulates the cell cycle while the desmosomal adhesion pathway maintains the structural cohesion between cells. PERP acts in concert with various proteins such as JUP (Plakoglobin) strengthening cellular adhesions and responding to apoptotic signals. This connectivity allows PERP to perform dual roles in maintaining cell stability and facilitating apoptosis when necessary.

PERP has associations with squamous cell carcinoma and epidermolysis bullosa. Disruption of PERP functions can lead to improper apoptosis contributing to cancer progression. In the case of epidermolysis bullosa alterations in desmosomal structure connected to PERP can weaken skin integrity leading to blistering. In these conditions proteins like JUP work alongside PERP highlighting the importance of balance between cell adhesion and controlled cell death in disease prevention.
style
left-column

Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

style
left-column

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

style
left-column

Product protocols

style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com