Human PEX5 (PER3) knockout HEK-293T cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human PEX5 (PER3) knockout HEK-293T cell lysate (AB263294)
Homozygous : Insertion of the selection cassette in exon3
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The PEX5 protein plays a significant role in peroxisomal protein import by participating in a transient multi-protein transport complex at the peroxisome membrane. This complex ensures that imported proteins are released into the peroxisomal matrix. PEX5 interacts closely with PEX14 a peroxisomal membrane protein to facilitate the docking of PTS1-carrying proteins. PEX5 is indispensable for maintaining normal peroxisomal function and integrity as it ensures that specific enzymes reach their destination to carry out metabolic processes.
Pathways
PEX5 interacts within the broader lipid metabolism and reactive oxygen species detoxification pathways. It contributes to the proper functioning of enzymes involved in beta-oxidation of fatty acids and plasmalogen synthesis. PEX5 operates in conjunction with the PEX7 receptor which handles the PTS2 pathway helping to manage the trafficking of enzymes important for peroxisomal operations thereby integrating lipid metabolism with cellular homeostasis.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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