Human PFN2 (Profilin 2) knockout HeLa cell lysate
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PFN2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2.
View Alternative Names
D3S1319E, PFL, PFN 2, PROF2_HUMAN, Profilin II, Profilin-2
- WB
Lab
Western blot - Human PFN2 (Profilin 2) knockout HeLa cell lysate (AB258108)
Lane 1 : Wild-type HeLa cell lysate 20 μg
Lane 2 : PFN2 knockout HeLa cell lysate 20 μg
Lane 3 : SH-SY5Y cell lysate 20 μg
Lane 4 : Daudi cell lysate 20 μg
False colour image of Western blot : Anti-Profilin 2 antibody [EPR11030] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab174322 was shown to bind specifically to Profilin 2. A band was observed at 13 kDa in wild-type HeLa cell lysates with no signal observed at this size in PFN2 knockout cell line ab265833 (knockout cell lysate ab258108). To generate this image, wild-type and PFN2 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-Profilin 2 antibody [EPR11030] (<a href='/en-us/products/primary-antibodies/profilin-2-antibody-epr11030-ab174322'>ab174322</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
PFN2 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human PFN2 (Profilin 2) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-pfn2-profilin-2-knockout-hela-cell-line-ab265833'>ab265833</a>)
Lane 3:
SH-SY5Y cell lysate at 20 µg
Lane 4:
Daudi cell lysate at 20 µg
Predicted band size: 15 kDa
Observed band size: 13 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human PFN2 (Profilin 2) knockout HeLa cell lysate (AB258108)
Homozygous : 1 bp insertion in exon2
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Profilin 2 regulates actin dynamics essential for cellular processes like membrane trafficking and signal transduction. It is not typically known to be part of an extensive protein complex but interacts with other proteins including members of the Arp2/3 complex to promote actin filament nucleation and branching. This regulation impacts processes like vesicle transport particularly in neuronal cells where Profilin 2 influences synaptic function and plasticity.
Pathways
Actin dynamics controlled by Profilin 2 play significant roles in both the Rho GTPase and PI3K/AKT signaling pathways. In these pathways Profilin 2 interfaces with Rho-related proteins to modulate the actin cytoskeleton impacting cell adhesion and movement. Its interaction with PI3K elements contributes to cell growth and survival signals aligning Profilin 2's functions with cellular responses to environmental cues.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
![Immunoprecipitation - Anti-Profilin 2 antibody [EPR11030] (AB174322)](https://content.abcam.com/products/images/profilin-2-antibody-epr11030-ab174322--immunoprecipitation-img74005.jpg)
Cell Lines & Lysates
AB265833
Human PFN2 (Profilin 2) knockout HeLa cell line
Advanced Validation
- 0
- 0
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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