Human PGAM5 knockout HeLa cell lysate
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(0 Publication)
- WB
Lab
Western blot - Human PGAM5 knockout HeLa cell lysate (AB257581)
Lane 1 : Wild-type HeLa cell lysate (20 μg)
Lane 2 : PGAM5 knockout HeLa cell lysate (20 μg)
Lane 3 : HepG2 cell lysate (20 μg)
Lane 4 : Daudi cell lysate (20 μg)
Lanes 1-4 : Merged signal (red and green). Green - ab244218 observed at 32 kDa. Red - loading control ab52866 observed at 50 kDa.
ab244218 Anti-PGAM5 antibody [CL0624] was shown to specifically react with PGAM5 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265141 (knockout cell lysate ab257581) was used. Wild-type and PGAM5 knockout samples were subjected to SDS-PAGE. ab244218 and Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab52866) were incubated overnight at 4°C at 1 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-PGAM5 antibody [CL0624] (<a href='/en-us/products/primary-antibodies/pgam5-antibody-cl0624-ab244218'>ab244218</a>) at 1 µg/mL
Lane 1:
Wild-type HeLa lysate at 20 µg
Lane 2:
PGAM5 knockout HeLa lysate at 20 µg
Lane 2:
Western blot - Human PGAM5 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-pgam5-knockout-hela-cell-line-ab265141'>ab265141</a>)
Lane 3:
HepG2 lysate at 20 µg
Lane 4:
Daudi lysate at 20 µg
Predicted band size: 32 kDa
Observed band size: 32 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human PGAM5 knockout HeLa cell lysate (AB257581)
Homozygous : 1 bp insertion in exon1
- WB
Supplier Data
Western blot - Human PGAM5 knockout HeLa cell lysate (AB257581)
All lanes:
Western blot - Anti-PGAM5 antibody [RM1254] (<a href='/en-us/products/primary-antibodies/pgam5-antibody-rm1254-ab322207'>ab322207</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2:
Western blot - Human PGAM5 knockout HeLa cell lysate (ab257581) at 20 µg
Lane 3:
A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4:
HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg
Lane 5:
MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution
Observed band size: 32 kDa,36 kDa
false
Complementary Products
Primary Antibodies
AB308449
Anti-PGAM5 antibody [EPR27145-82]
20ul selling size|RabMAb|Recombinant|KO Validated
![Immunocytochemistry/ Immunofluorescence - Anti-PGAM5 antibody [EPR27145-82] (AB308449)](https://content.abcam.com/products/images/pgam5-antibody-epr27145-82-ab308449--immunocytochemistry-immunofluorescence-img409821.jpg)
- 0
- 0
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PGAM5 impacts pathways related to cellular stress responses and energy metabolism. It participates in programmed cell death and modulates mitochondrial dynamics. It can serve as part of a complex with other proteins adjusting the balance between mitophagy and apoptosis. Through interaction with key signaling molecules PGAM5 bridges various biochemical processes relevant to cell survival and energy utilization.
Pathways
Its function integrates into mitophagy and necroptosis. PGAM5 collaborates with proteins like PINK1 and PARKIN contributing to the clearance of damaged mitochondria via the mitophagy pathway. In necroptosis PGAM5 interacts with RIPK1 and RIPK3 regulating necrotic cell death. These interactions position PGAM5 at the crossroads of pathways critical to maintaining cellular homeostasis and response to stress.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com