Human PHF11 knockout A549 cell lysate
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PHF11 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon6.
View Alternative Names
APY, BCAP, BRCA1 C-terminus-associated protein, IGEL, IGER, IGHER, IgE responsiveness (atopic), NY REN 34 antigen, NY-REN-34, PHD finger protein 11, PHF11_HUMAN, RP11-185C18.3, Renal carcinoma antigen NY-REN-34
- Sanger seq
Unknown
Sanger Sequencing - Human PHF11 knockout A549 cell lysate (AB258578)
Homozygous : 2 bp deletion in exon6
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PHF11 regulates the immune response and chromatin-mediated transcription. Its role involves influencing the accessibility of chromatin for transcriptional machinery aiding in gene expression modulation. Although it primarily functions alone PHF11 can also associate with chromatin remodeling complexes to fulfill its role in transcription regulation. It contributes to cytokine expression impacting immune response modulation which is critical for organismal defense mechanisms.
Pathways
PHF11 participates in immune response pathways including the adaptive immune response. It interacts with other proteins in these pathways such as transcription factors and histones to mediate transcription of immune-related genes. An important relationship exists with proteins involved in the NF-kB signaling pathway which is essential for immune and inflammatory responses. Through its interactions PHF11 helps fine-tune immune gene activation and contributes to balancing immune signaling.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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