PHIP KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 1 bp insertion in exon1.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 1 bp insertion in exon1.
Alternative names
DCAF14, DDB1 and CUL4 associated factor 14, FLJ20705, FLJ45918, IRS 1 PH domain binding protein, MGC90216, Ndrp, Neuronal differentiation related protein, PH interacting protein, Pleckstrin homology domain interacting protein, WD repeat containing protein 11, WD repeat protein 11
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PHIP KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 1 bp insertion in exon1.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 1 bp insertion in exon1.
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- PHIP
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PHIP also known as pleckstrin homology domain interacting protein is an adapter protein that interacts with insulin receptor substrates. It has a mass of approximately 100 kDa. PHIP is expressed in various tissues including the brain and liver. The protein is involved in intracellular signaling pathways by binding phosphoinositides which are important for cellular communication and growth regulation.
- Biological function summary
PHIP plays a significant role in cell proliferation and survival. It contributes to the regulation of the cell cycle by influencing transcription factors and other core components involved in cell growth. PHIP is not only a standalone protein but also part of complexes that modulate chromatin affecting gene expression patterns.
- Pathways
PHIP is integrated into insulin signaling and PI3K/AKT pathways. In these pathways PHIP interacts with key proteins like AKT1 supporting cellular responses to insulin and growth factors. The involvement of PHIP in these pathways highlights its role in metabolic regulation and its impact on cell growth and metabolism.
- Associated diseases and disorders
PHIP has links to cancer and diabetes. Its dysregulation contributes to abnormal cell proliferation often observed in cancers. PHIP's interaction with insulin signaling components such as IRS1 demonstrates its relevance to diabetes management. These connections show PHIP's potential as a therapeutic target for these conditions.
Product promise
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Terms & Conditions.
3 product images
Sanger Sequencing - Human PHIP knockout HeLa cell lysate (ab259039)
Allele-1: 1 bp deletion in exon1
Sanger Sequencing - Human PHIP knockout HeLa cell lysate (ab259039)
Allele-2: 1 bp insertion in exon1
Western blot - Human PHIP knockout HeLa cell lysate (ab259039)
All lanes: Western blot - Anti-PHIP antibody [EPR27150-82] (Anti-PHIP antibody [EPR27150-82] ab309962) at 1/1000 dilution
Lane 1: Wild-type HeLa(human cervical adenocarcinoma epithelial cell) whole cell lysate at 40 µg
Lane 2: Western blot - Human PHIP knockout HeLa cell lysate (ab259039) at 40 µg
Lane 3: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 250 kDa
Exposure time: 3min
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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