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AB258111

Human PHKA1 knockout HEK-293T cell lysate

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PHKA1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon14.
2 Images
Western blot - Human PHKA1 knockout HEK-293T cell lysate (AB258111)
  • WB

Lab

Western blot - Human PHKA1 knockout HEK-293T cell lysate (AB258111)

Lanes 1 - 2:

Western blot - Anti-PHKA1 antibody [EPR12118] - BSA and Azide free (<a href='/en-us/products/primary-antibodies/phka1-antibody-epr12118-bsa-and-azide-free-ab249913'>ab249913</a>) at 1/1000 dilution

Lanes 1 - 2:

Western blot - Anti-PHKA1 antibody [EPR12118] (<a href='/en-us/products/primary-antibodies/phka1-antibody-epr12118-ab176338'>ab176338</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

PHKA1 knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human PHKA1 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-phka1-knockout-hek-293t-cell-line-ab267337'>ab267337</a>)

Lane 2:

Western blot - Human PHKA1 knockout HEK-293T cell lysate (ab258111)

Secondary

All lanes:

Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution

Observed band size: 130 kDa

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Sanger Sequencing - Human PHKA1 knockout HEK-293T cell lysate (AB258111)
  • Sanger seq

Unknown

Sanger Sequencing - Human PHKA1 knockout HEK-293T cell lysate (AB258111)

Homozygous : 2 bp deletion in exon14

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon14.

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Complementary Products

Primary Antibodies

AB176338

Anti-PHKA1 antibody [EPR12118]

RabMAb|Recombinant|KO Validated

Western blot - Anti-PHKA1 antibody [EPR12118] (AB176338)

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Cell Lines & Lysates

AB267337

Human PHKA1 knockout HEK-293T cell line

Western blot - Human PHKA1 knockout HEK-293T cell line (AB267337)

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab258111-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human PHKA1 knockout HEK293T cell lysate", "number":"AB258111-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HEK293T cell lysate", "number":"AB258111-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Gene name
PHKA1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PHKA1 also known as phosphorylase kinase alpha 1 is a regulatory subunit of the phosphorylase kinase enzyme complex with a molecular mass around 138 kDa. This protein is mainly expressed in skeletal muscle and heart tissue. PHKA1 plays an important role in glycogen metabolism by regulating the conversion of phosphorylase b to phosphorylase a which ultimately leads to glycogen breakdown.
Biological function summary

Glycogen metabolism involves PHKA1 as part of the phosphorylase kinase complex. This complex exists in the muscle and liver and includes alpha beta gamma and delta subunits. PHKA1 binds together with these subunits to form a large complex helping to regulate the activity state of the enzyme. The regulation of glycogen phosphorylase is important for maintaining energy supply in cells particularly during physical activity.

Pathways

The activity of PHKA1 impacts glycogenolysis which is a significant metabolic pathway responsible for breaking down glycogen into glucose-1-phosphate. This pathway involves other proteins like glycogen phosphorylase and phosphoprotein phosphatase 1. PHKA1 has interactions within the cascade that ensures proper glycogen breakdown especially during conditions requiring increased energy output.

Mutations in PHKA1 are linked to glycogen storage disease type IXd (GSD IXd) which primarily affects muscle tissue. This condition features exercise intolerance and muscle weakness due to impaired glycogen breakdown. PHKB another homologous phosphorylase kinase subunit can connect to similar energy metabolism disruptions showing a clear association with the disorder pathway alongside PHKA1.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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