Human PI4K2A knockout HeLa cell lysate
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PI4K2A KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 2 bp deletion in exon1.
View Alternative Names
P4K2A_HUMAN, PI4KII, Phosphatidylinositol 4-kinase type 2-alpha, Phosphatidylinositol 4-kinase type II-alpha
- Sanger seq
Unknown
Sanger Sequencing - Human PI4K2A knockout HeLa cell lysate (AB258580)
Allele-2 : 1 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human PI4K2A knockout HeLa cell lysate (AB258580)
Allele-1 : 2 bp deletion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PI4K2A plays a significant role in cellular processes through its involvement in phosphoinositide turnover. It contributes to the synthesis of membrane phospholipids and helps regulate vesicle trafficking. PI4K2A functions as part of larger protein complexes involved in generating PI4P a precursor for producing phosphatidylinositol 45-bisphosphate (PI(45)P2) which is critical for various signaling pathways.
Pathways
PI4K2A associates with the phosphoinositide signaling pathway and the endocytic pathway. In the phosphoinositide signaling pathway it interacts with other kinases to facilitate the production of PI4P and PI(45)P2 which serve as signaling molecules. Within these pathways PI4K2A collaborates with proteins like PI4KB to regulate membrane dynamics and endocytosis.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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