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AB258581

Human PI4K2B knockout HeLa cell lysate

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PI4K2B KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.
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Sanger Sequencing - Human PI4K2B knockout HeLa cell lysate (AB258581)
  • Sanger seq

Unknown

Sanger Sequencing - Human PI4K2B knockout HeLa cell lysate (AB258581)

Homozygous : 1 bp insertion in exon 1

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.

Disease

Adenocarcinoma

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Complementary Products

Proteins & Peptides

AB268861

Recombinant human PI4K2B protein (Active)

Functional Studies - Recombinant human PI4K2B protein (Active) (AB268861)

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Cell Lines & Lysates

AB264683

Human PI4K2B knockout HeLa cell line

Sanger Sequencing - Human PI4K2B knockout HeLa cell line (AB264683)

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  • 0
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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
PI4K2B
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Zygosity
Homozygous
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PI4K2B also known as Phosphatidylinositol 4-kinase type 2 beta is an enzyme with a molecular mass of approximately 55 kDa. This protein plays a role in the phosphorylation of phosphatidylinositol converting it to phosphatidylinositol 4-phosphate (PI4P) an important step in the phosphatidylinositol signaling pathway. PI4K2B shows expression in a variety of human tissues with particularly high levels noted in the placenta and fibroblasts. Its activity influences the formation of different cellular membranes impacting various cellular processes.
Biological function summary

PI4K2B influences multiple cellular functions particularly those related to membrane trafficking and cell signaling. Although not part of a larger known complex PI4K2B's enzymatic production of PI4P has many downstream effects impacting endosomal dynamics and vesicular trafficking. The protein's involvement in membrane dynamics suggests important roles in regulating cell movement and recycling of membrane components affecting how cells respond to their surroundings.

Pathways

PI4K2B integrates into the broader phosphoinositide pathway that regulates cellular communication and processes. It closely interacts with proteins like AKT and RAB proteins which are essential in the regulation of vesicle transport and signal transduction. The production of PI4P by PI4K2B contributes to the modulation of the PI3K/AKT signaling pathway impacting cellular growth survival and metabolism. This pathway's complexity demonstrates PI4K2B's role in coordinating multiple biological reactions.

Studies link altered PI4K2B function to neurodegenerative diseases and cancer. Its aberrant activity influences the signaling pathways that contribute to the development and progression of these conditions. In the context of neurodegeneration PI4K2B interacts with proteins such as tau influencing the balance of endosomal transport in neurons. In cancer changes in its activity affect cellular proliferation and survival showing connections to other oncogenic proteins within signaling cascades. These implications highlight the importance of understanding PI4K2B's roles in health and disease.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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