Human PICALM knockout U-2 OS cell lysate
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PICALM KO cell lysate available now. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9.
View Alternative Names
CLTH, Clathrin assembly lymphoid myeloid leukemia, Clathrin assembly lymphoid myeloid leukemia protein, LAP, PICAL_HUMAN, Phosphatidylinositol-binding clathrin assembly protein
- WB
Lab
Western blot - Human PICALM knockout U-2 OS cell lysate (AB263930)
Lane 1 : Wild-type U-2 OS whole cell lysate 20 ug
Lane 2 : PICALM knockout U-2 OS whole cell lysate 20 ug
Lane 3 : PC3 (Human prostate adenocarcinoma cell line) whole cell lysate 20 ug
Lane 4 : Caco-2 (Human colorectal adenocarcinoma cell line) whole cell lysate 20 ug
Lanes 1 - 4 : Merged signal (red and green). Green - ab172962 observed at 70-75 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.
ab172962 was shown to react with PICALM in U-2 OS wild-type cells in Western blot. Loss of signal was observed when PICALM knockout cell line ab262500 (knockout cell lysate ab263930) was used. Wild-type U-2 OS and PICALM knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab172962 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 5000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-PICALM antibody [EPR12177] (<a href='/en-us/products/primary-antibodies/picalm-antibody-epr12177-ab172962'>ab172962</a>) at 1/5000 dilution
Lane 1:
Wild-type U-2 OS whole cell lysate at 20 µg
Lane 2:
PICALM knockout U-2 OS whole cell lysate at 20 µg
Lane 2:
Western blot - Human PICALM knockout U-2 OS cell line (<a href='/en-us/products/cell-lines/human-picalm-knockout-u-2-os-cell-line-ab262500'>ab262500</a>)
Lane 3:
PC3 (Human prostate adenocarcinoma cell line) whole cell lysate at 20 µg
Lane 4:
Caco-2 (Human colorectal adenocarcinoma cell line) whole cell lysate at 20 µg
Predicted band size: 70 kDa
Observed band size: 70-75 kDa
false
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PICALM is essential in the trafficking of molecules within cells particularly in the endocytosis and endosomal sorting pathways. This protein is part of large protein complexes assembled during the formation of vesicles. Through these complexes PICALM influences the transport and recycling of membrane receptors and other molecules ensuring proper cellular functions. The clathrin coat assembly and disassembly rely heavily on its function for effective cellular trafficking processes.
Pathways
PICALM integrates into the clathrin-mediated endocytosis and vesicle-mediated transport pathways. It works alongside proteins such as adaptin and dynamin to facilitate the internalization of proteins and later processing within cellular compartments. In the context of cellular signaling and homeostasis these pathways involving PICALM contribute significantly to receptor recycling and downregulation maintaining proper cellular responses to external stimuli.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Female
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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