Human PIGT knockout HEK-293T cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human PIGT knockout HEK-293T cell lysate (AB263295)
Allele-1 : 5 bp deletion in exon 1
- Sanger seq
Unknown
Sanger Sequencing - Human PIGT knockout HEK-293T cell lysate (AB263295)
Allele-2 : 1 bp deletion in exon 1
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PIGT acts as a subunit within the GPI transamidase complex that enhances the transfer of pre-assembled GPI units to proteins. This activity anchors proteins to the cell surface which is necessary for their stability and function. The GPI anchor allows effective signaling adhesion and protection of proteins impacting many cellular processes. The interdependency with other subunits such as PIGA PIGK and PIGS ensures the complete function of the GPI transamidase complex.
Pathways
PIGT remains key within the GPI-anchor biosynthesis pathway playing a critical role in cell surface protein localization. It interacts with other members like PIGU and GAA1 within this pathway forming interactions necessary for proper transit and attachment of GPI-anchored proteins. The functionality of the GPI-anchor pathway directly influences cellular communication through pathways like signal transduction which are essential for different biological processes.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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