PIK3C2A KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 43 bp insertion in exon 1.
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Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 43 bp insertion in exon 1.
Alternative names
C2 containing phosphatidylinositol kinase, C2 domain containing alpha polypeptide, CPK, Class 2 alpha polypeptide, Cpk m, DKFZp686L193, MGC142218, OTTHUMP00000231763, P3C2A_HUMAN, PI3-K-C2(ALPHA), PI3-K-C2A, PI3K-C2-alpha, PIK3C 2A, Phosphatidylinositol 3 kinase class 2 alpha, Phosphatidylinositol-4-phosphate 3-kinase C2 domain-containing subunit alpha, Phosphoinositide 3 kinase class 2 alpha, Phosphoinositide 3 kinase, class 2, alpha polypeptide, Phosphoinositide 3-Kinase-C2-alpha, PtdIns-3-kinase C2 subunit alpha
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PIK3C2A KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 43 bp insertion in exon 1.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 43 bp insertion in exon 1.
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- PIK3C2A
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
- Zygosity
- Homozygous
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PI 3 Kinase Class 2A also known as Cpk is a significant enzyme involved in the regulation of various cellular processes. The target weighing approximately 170 kDa acts as a lipid kinase that phosphorylates phosphatidylinositol converting PI to PI3P. Cpk expresses mainly in tissues like the liver muscle and heart where it contributes to cellular homeostasis and metabolic regulation.
- Biological function summary
This enzyme family modulates intracellular signaling cascades influencing processes such as cell growth survival and metabolism. Cpk forms part of a larger complex that interacts with adaptor proteins facilitating its recruitment to cellular membranes. This interaction is important for its signaling activity and function in maintaining cellular equilibrium.
- Pathways
PI 3 Kinase Class 2A plays an integral role in the insulin signaling pathway and the TOR signaling pathway. In the insulin pathway it collaborates with proteins like Akt to modulate glucose uptake and storage in cells. Similarly in the TOR pathway Cpk contributes to nutrient sensing and cell growth regulation interconnected with proteins such as mTOR affecting protein synthesis and autophagy.
- Associated diseases and disorders
Dysregulation of PI 3 Kinase Class 2A links to cancer and type 2 diabetes. Alterations in its activity may lead to abnormal cell proliferation and survival characteristics of cancerous transformations associated with proteins like PTEN. Meanwhile its role in glucose metabolism ties it to insulin resistance and diabetes pathogenesis where interactions with insulin receptor substrates become important for disease progression.
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1 product image
Sanger Sequencing - Human PIK3C2A (PI 3 Kinase Class 2A/Cpk) knockout HeLa cell lysate (ab259040)
Homozygous: 43 bp insertion in exon 1
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