JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB257586

Human PIK3R2 (PI 3 Kinase p85 beta) knockout HEK-293T cell lysate

Be the first to review this product! Submit a review

|

(0 Publication)

PIK3R2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2.

View Alternative Names

P85B_HUMAN, PI3 kinase p85 beta subunit, PI3 kinase p85 subunit beta, PI3-kinase regulatory subunit beta, PI3-kinase subunit p85-beta, PI3K, PI3K regulatory subunit beta, PIK3R 2, Phosphatidylinositol 3 kinase, Phosphatidylinositol 3 kinase regulatory beta subunit, Phosphatidylinositol 3 kinase regulatory subunit polypeptide 2, Phosphatidylinositol 3 kinase, regulatory subunit, polypeptide 2 (p85 beta), Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta, Phosphatidylinositol 3-kinase regulatory subunit beta, Phosphoinositide 3 kinase regulatory subunit 2, Phosphoinositide 3 kinase regulatory subunit 2 (beta), Phosphoinositide 3 kinase regulatory subunit polypeptide 2, Phosphoinositide 3 kinase regulatory subunit polypeptide 2 (p85 beta), Phosphoinositide 3 kinase, regulatory subunit 2 (p85 beta), PtdIns 3 kinase p85 beta, PtdIns-3-kinase regulatory subunit beta, PtdIns-3-kinase regulatory subunit p85-beta, p85, p85 beta

3 Images
Western blot - Human PIK3R2 (PI 3 Kinase p85 beta) knockout HEK-293T cell lysate (AB257586)
  • WB

Unknown

Western blot - Human PIK3R2 (PI 3 Kinase p85 beta) knockout HEK-293T cell lysate (AB257586)

Lane 1 : Wild-type HEK-293T cell lysate (20µg)

Lane 2 : PIK3R2 knockout HEK-293T cell lysate (20µg)

Lanes 1- 2 : Merged signal (red and green). Green - ab28356 observed at 85 kDa. Red - loading control ab181602 observed at 37 kDa.

ab28356 Anti-PI 3 Kinase p85 beta antibody [T15] was shown to specifically react with PI 3 Kinase p85 beta in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266799 (knockout cell lysate ab257586) was used. Wild-type and PI 3 Kinase p85 beta knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab28356 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PI 3 Kinase p85 beta antibody [T15] (<a href='/en-us/products/primary-antibodies/pi-3-kinase-p85-beta-antibody-t15-ab28356'>ab28356</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

PIK3R2 knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human PIK3R2 (PI 3 Kinase p85 beta) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-pik3r2-pi-3-kinase-p85-beta-knockout-hek-293t-cell-line-ab266799'>ab266799</a>)

Predicted band size: 81 kDa

Observed band size: 85 kDa

false

Western blot - Human PIK3R2 (PI 3 Kinase p85 beta) knockout HEK-293T cell lysate (AB257586)
  • WB

Lab

Western blot - Human PIK3R2 (PI 3 Kinase p85 beta) knockout HEK-293T cell lysate (AB257586)

Lane 1 : Wild-type HEK-293T cell lysate (20µg)

Lane 2 : PIK3R2 knockout HEK-293T cell lysate (20µg)

Lanes 1- 2 : Merged signal (red and green). Green - ab180967 observed at 85 kDa. Red - loading control ab8245 observed at 37 kDa.

ab180967 Anti-PI 3 Kinase p85 beta antibody [EPR18416] was shown to specifically react with PI 3 Kinase p85 beta in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266799 (knockout cell lysate ab257586) was used. Wild-type and PI 3 Kinase p85 beta knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab180967 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 2000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PI 3 Kinase p85 beta antibody [EPR18416] (<a href='/en-us/products/primary-antibodies/pi-3-kinase-p85-beta-antibody-epr18416-ab180967'>ab180967</a>) at 1/2000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

PIK3R2 knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human PIK3R2 (PI 3 Kinase p85 beta) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-pik3r2-pi-3-kinase-p85-beta-knockout-hek-293t-cell-line-ab266799'>ab266799</a>)

Predicted band size: 56 kDa,81 kDa

Observed band size: 56 kDa,85 kDa

false

Sanger Sequencing - Human PIK3R2 (PI 3 Kinase p85 beta) knockout HEK-293T cell lysate (AB257586)
  • Sanger seq

Unknown

Sanger Sequencing - Human PIK3R2 (PI 3 Kinase p85 beta) knockout HEK-293T cell lysate (AB257586)

Homozygous : 1 bp insertion in exon 2

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 2.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

style
left-column

What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab257586-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human PIK3R2 knockout HEK293T cell lysate", "number":"AB257586-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HEK293T cell lysate", "number":"AB257586-CMP02", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Gene name
PIK3R2
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Zygosity
Homozygous
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PI 3-kinase p85 beta also known as PIK3R2 is a regulatory subunit of phosphoinositide 3-kinases (PI3K). This protein has a molecular weight of approximately 85 kilodaltons. It expresses widely in various tissues including brain liver and skeletal muscle. The p85 protein form interacts with the catalytic subunit p110 allowing for the proper localization and activation of PI3K in response to various extracellular signals.
Biological function summary

The regulatory subunit PI 3-kinase p85 beta is responsible for maintaining the balance of PI3K activity within the cellular environment. It forms a dynamic complex with the catalytic subunit influencing downstream signaling pathways. The presence of p85 beta ensures the stability and function of the PI3K complex essential for processes such as cell growth survival and metabolism.

Pathways

This protein plays a significant role in the PI3K/AKT/mTOR signaling pathway and also contributes to insulin signaling. It partners with p110 and mediates the transmission of signals from receptor tyrosine kinases (RTKs) like insulin receptors. The regulation by p85 beta affects protein synthesis and glucose uptake highlighting its function in cellular energy management.

Dysregulation of PI 3-kinase p85 beta associates frequently with types of cancer and type 2 diabetes mellitus. Aberrant signaling through the PI3K pathway involving interactions between p85 beta and p110 contributes to oncogenesis. Similarly inappropriate regulation of insulin signaling links p85 beta with insulin resistance an important factor in type 2 diabetes where it may interact with proteins such as IRS-1 (Insulin Receptor Substrate 1) to influence disease progression.
style
left-column

Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

style
left-column

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

style
left-column

Product protocols

style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB180967

Anti-PI 3 Kinase p85 beta antibody [EPR18416]

RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PI 3 Kinase p85 beta antibody [EPR18416] (AB180967)

  • 0
  • 0
Proteins & Peptides

AB259412

Recombinant human VEGF 165A protein (Active)

Premium Bioactive

Mass Spectrometry - Recombinant human VEGF 165A protein (Active) (AB259412)

  • 0
  • 0
Proteins & Peptides

AB288799

Recombinant Human VCAM1 protein (Active)

Premium Bioactive

Biological Activity - Recombinant Human VCAM1 protein (Active) (AB288799)

  • 0
  • 0
Primary Antibodies

AB40776

Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EP383Y]

20ul selling size|RabMAb|Recombinant|KO Validated

Western blot - Anti-PI 3 Kinase catalytic subunit alpha/PIK3CA antibody [EP383Y] (AB40776)

  • 0
  • 1

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com