JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB263298

Human PIP5K1A knockout HEK-293T cell lysate

Be the first to review this product! Submit a review

|

(0 Publication)

PIP5K1A KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 5.

View Alternative Names

68 kDa type I phosphatidylinositol-4-phosphate 5-kinase alpha, PI4P5K Ia, PI51A_HUMAN, PIP5K A, PIP5K1-alpha, PIP5KIalpha, Phosphatidylinositol-4-phosphate 5-kinase type I alpha, Phosphatidylinositol-4-phosphate 5-kinase type-1 alpha, Pip5k1a, PtdIns(4)P-5-kinase 1 alpha

1 Images
Sanger Sequencing - Human PIP5K1A knockout HEK-293T cell lysate (AB263298)
  • Sanger seq

Unknown

Sanger Sequencing - Human PIP5K1A knockout HEK-293T cell lysate (AB263298)

Homozygous : 1 bp insertion in exon 5

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 5.

style
left-column

Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

style
left-column

What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab263298-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human PIP5K1A knockout HEK293T cell lysate", "number":"AB263298-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HEK293T cell lysate", "number":"AB263298-CMP02", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Gene name
PIP5K1A
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Zygosity
Homozygous
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PIP5K1 alpha also known as Phosphatidylinositol-4-phosphate 5-kinase type 1 alpha (PIP5K1A) is an enzyme that catalyzes the phosphorylation of phosphatidylinositol-4-phosphate (PI4P) to generate phosphatidylinositol-45-bisphosphate (PI(45)P2). The protein has an approximate mass of 68 kDa and is highly expressed in a variety of human tissues including the brain heart and spleen. PIP5K1A plays a significant role in lipid signaling and membrane dynamics impacting a wide array of cellular processes such as cytoskeletal organization and vesicle trafficking.
Biological function summary

PIP5K1A influences the production of PI(45)P2 an important phosphoinositide involved in numerous cellular functions. PI(45)P2 acts as a substrate for the production of secondary messengers like inositol trisphosphate (IP3) and diacylglycerol (DAG) essential in signal transduction pathways. PIP5K1A is part of a larger protein complex interacting with cytoskeletal elements and regulators of endocytosis and exocytosis. This association highlights its importance in maintaining cellular compartmentalization and function.

Pathways

PIP5K1A plays a pivotal role in the phosphoinositide signaling pathway and the actin cytoskeleton regulation pathway. It works closely with proteins such as RAC1 a small GTPase involved in actin polymerization to facilitate changes in the cell structure and motility. Additionally PIP5K1A's product PI(45)P2 serves as a precursor for multiple signaling molecules integrating with pathways that mediate cellular responses to various extracellular stimuli.

PIP5K1A's dysregulation links to cancer and neurological disorders. In cancer alterations in PIP5K1A expression or activity can affect cell proliferation and survival interacting with proteins like AKT that drive oncogenic signaling. In the context of neurological disorders its role in synaptic function and plasticity directly connects to conditions like schizophrenia where PI(45)P2 imbalance may contribute to the disorder's pathophysiology. These associations highlight the essential impact of PIP5K1A in maintaining cellular homeostasis and its potential as a therapeutic target.
style
left-column

Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

style
left-column

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

style
left-column

Product protocols

style
left-column
style
left-column
style
right-column

Complementary Products

Cell Lines & Lysates

AB257349

Human ALKBH5 knockout HEK-293T cell lysate

Western blot - Human ALKBH5 knockout HEK-293T cell lysate (AB257349)

  • 0
  • 0
Primary Antibodies

AB189491

Anti-Heme Oxygenase 1 antibody [EPR18161-128]

RabMAb|Recombinant|KO Validated

Western blot - Anti-Heme Oxygenase 1 antibody [EPR18161-128] (AB189491)

  • 5
  • 2
Proteins & Peptides

AB261922

Recombinant human PIP5K1 alpha/PIP5K1A protein (Tagged)

Functional Studies - Recombinant human PIP5K1 alpha/PIP5K1A protein (Tagged) (AB261922)

  • 0
  • 0
Primary Antibodies

AB62352

Anti-Nrf2 antibody [EP1808Y]

RabMAb|Recombinant|KO Validated

Western blot - Anti-Nrf2 antibody [EP1808Y] (AB62352)

  • 4
  • 33

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com