JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB258117

Human PIP5K1C knockout HeLa cell lysate

Be the first to review this product! Submit a review

|

(0 Publication)

PIP5K1C KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon4 and 5 bp deletion in exon4.
3 Images
Western blot - Human PIP5K1C knockout HeLa cell lysate (AB258117)
  • WB

Supplier Data

Western blot - Human PIP5K1C knockout HeLa cell lysate (AB258117)

Lane 1 : Wild-type HeLa cell lysate (20 μg)

Lane 2 : PIP5K1C knockout HeLa cell lysate (20 μg)

Lane 3 : Neuro-2a cell lysate (20 μg)

Lane 4 : A549 cell lysate (20 μg)

Lanes 1-4 : Merged signal (red and green). Green - ab109192 observed at 73 kDa. Red - loading control ab8245 observed at 37 kDa.

ab109192 Anti-PIP5K1C antibody [MAO-R1] was shown to specifically react with PIP5K1C in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265545 (knockout cell lysate ab258117) was used. Wild-type and PIP5K1C knockout samples were subjected to SDS-PAGE. ab109192 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PIP5K1C antibody [MAO-R1] (<a href='/en-us/products/primary-antibodies/pip5k1c-antibody-mao-r1-ab109192'>ab109192</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

PIP5K1C knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human PIP5K1C knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-pip5k1c-knockout-hela-cell-line-ab265545'>ab265545</a>)

Lane 3:

Neuro-2a cell lysate at 20 µg

Lane 4:

A549 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Predicted band size: 73 kDa

Observed band size: 73 kDa

false

Sanger Sequencing - Human PIP5K1C knockout HeLa cell lysate (AB258117)
  • Sanger seq

Unknown

Sanger Sequencing - Human PIP5K1C knockout HeLa cell lysate (AB258117)

Allele-2 : 1 bp insertion in exon4

Sanger Sequencing - Human PIP5K1C knockout HeLa cell lysate (AB258117)
  • Sanger seq

Unknown

Sanger Sequencing - Human PIP5K1C knockout HeLa cell lysate (AB258117)

Allele-1 : 5 bp deletion in exon4

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon4 and 5 bp deletion in exon4.

Disease

Adenocarcinoma

style
left-column

Complementary Products

Proteins & Peptides

AB268872

Recombinant human PIP5K1C protein (Active)

Functional Studies - Recombinant human PIP5K1C protein (Active) (AB268872)

  • 0
  • 0
Primary Antibodies

AB109192

Anti-PIP5K1C antibody [MAO-R1]

RabMAb|Recombinant|KO Validated

Western blot - Anti-PIP5K1C antibody [MAO-R1] (AB109192)

  • 0
  • 0
Proteins & Peptides

AB259412

Recombinant human VEGF 165A protein (Active)

Premium Bioactive

Mass Spectrometry - Recombinant human VEGF 165A protein (Active) (AB259412)

  • 0
  • 0
Cell Lines & Lysates

AB265545

Human PIP5K1C knockout HeLa cell line

Advanced Validation

Western blot - Human PIP5K1C knockout HeLa cell line (AB265545)

  • 0
  • 0
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

style
left-column

What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab258117-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human PIP5K1C knockout HeLa cell lysate", "number":"AB258117-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HeLa cell lysate", "number":"AB258117-CMP02", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Gene name
PIP5K1C
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein PIP5K1C also known as phosphatidylinositol-4-phosphate 5-kinase type-1 gamma has a mass of approximately 75 kDa. This enzyme plays a critical role in the phosphatidylinositol signaling pathway where it catalyzes the phosphorylation of phosphatidylinositol 4-phosphate (PI4P) to produce phosphatidylinositol 45-bisphosphate (PIP2). PIP5K1C's activity is pivotal for membrane trafficking and it demonstrates widespread expression in tissues including the brain and skeletal muscle.
Biological function summary

PIP5K1C is involved in the regulation of actin cytoskeleton dynamics. It participates as an important enzyme in complexes that modulate actin polymerization and cell adhesion processes. Its function impacts cell motility and maintenance of cell shape contributing significantly to endocytosis and exocytosis. This function highlights the enzyme's role in intracellular signaling and adhesion-related activities.

Pathways

PIP5K1C interacts intricately within the phosphoinositide signaling pathway and impacts the regulation of focal adhesion. It influences other proteins like RAC1 an important regulator of actin polymerization and talin which is involved in linking integrins to the actin cytoskeleton. These interactions place PIP5K1C as an important contributor to the molecular mechanisms that control cell movement and attachment.

Mutations or dysfunctions in PIP5K1C have been associated with neurological disorders such as Charcot-Marie-Tooth disease type 4F and L-fucosidosis. Abnormalities in PIP5K1C expression or activity can disrupt cellular functions and lead to these conditions. In the context of Charcot-Marie-Tooth disease PIP5K1C may interact with dynamin 2 a protein involved in endocytic vesicle fission which further influences the peripheral nervous system pathology.
style
left-column

Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

style
left-column

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

style
left-column

Product protocols

style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com