Human PITPNB knockout HeLa cell lysate
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PITPNB KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and Insertion of the selection cassette in exon 2.
View Alternative Names
OTTHUMP00000198846, OTTHUMP00000198847, PI-TP-beta, PIPNB_HUMAN, Phosphatidylinositol transfer protein beta isoform, Phosphatidylinositol transfer protein, beta, PtdIns transfer protein beta, PtdInsTP, PtdInsTP beta, VIB1B
- Sanger seq
Unknown
Sanger Sequencing - Human PITPNB knockout HeLa cell lysate (AB259042)
Allele-2 : Insertion of the selection cassette in exon 2
- Sanger seq
Unknown
Sanger Sequencing - Human PITPNB knockout HeLa cell lysate (AB259042)
Allele-1 : 1 bp deletion in exon 2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PITPNB ensures the proper distribution of lipids within cells for membrane composition and signaling activities. It participates in maintaining the balance of lipid composition among cell membranes. PITPNB does not function alone; it often acts as part of lipid transfer complexes collaborating with other proteins to execute its function properly.
Pathways
PITPNB plays an active role in phosphoinositide signaling and membrane trafficking pathways. These pathways are critical for cell signaling and membrane dynamics. PI4K2A and PI4KA are proteins related to these pathways and interact with PITPNB highlighting its contribution to complex intracellular signaling processes.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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