Human PKDCC (VLK) knockout HeLa cell lysate
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PKDCC KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 32 bp deletion in exon1.
View Alternative Names
FLJ18197, Hypothetical protein BC007901, Hypothetical protein LOC91461, MGC125960, PKDCC_HUMAN, Protein kinase domain containing cytoplasmic, Protein kinase domain containing, cytoplasmic homolog (mouse), Protein kinase domain-containing protein, cytoplasmic, Protein kinase-like protein SgK493, SGK493, Sugen kinase 493, Vertebrate lonesome kinase
- Sanger seq
Unknown
Sanger Sequencing - Human PKDCC (VLK) knockout HeLa cell lysate (AB259043)
Homozygous : 32 bp deletion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
VLK kinase activity impacts protein folding and trafficking within the Golgi apparatus. VLK associates with protein complexes involved in secretory pathways assisting cellular functions like protein secretion and cell surface expression. Research indicates that it contributes significantly to maintaining cellular homeostasis by ensuring proper protein maturation.
Pathways
VLK plays a role in the secretory pathway which is essential for the transport and delivery of proteins and lipids throughout the cell. It shows interactions with proteins like ABCD3 and VCP that are pivotal in vesicular transport and protein quality control. VLK also participates in the RAS signaling pathway linking it to cell growth and differentiation by affecting downstream targets.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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