Human PKN2 knockout HCT116 cell lysate
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PKN2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon5 and 23 bp deletion in exon5.
View Alternative Names
Cardiolipin activated protein kinase Pak2, MGC150606, MGC71074, PKN gamma, PKN2_HUMAN, PRK2, PRKCL2, PRO2042, Protein kinase C-like 2, Protein kinase N2, Protein-kinase C-related kinase 2, Serine/threonine-protein kinase N2
- WB
Lab
Western blot - Human PKN2 knockout HCT116 cell lysate (AB258588)
Lane 1 : Wild-type HCT 116 (Human colorectal carcinoma cell line) whole cell lysate (20 ug)
Lane 2 : PKN2 knockout HCT 116 (Human colorectal carcinoma cell line) whole cell lysate (20 ug)
Lane 3 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (20 ug)
ab138514 was shown to specifically react with PKN2 in wild-type HCT cells. Loss of signal was observed when knockout cell line ab266894 (knockout cell lysate ab258588) was used. Wild-type and PKN2 knockout samples were subjected to SDS-PAGE. ab138514 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-PKN2 antibody [EPR5490] (<a href='/en-us/products/primary-antibodies/pkn2-antibody-epr5490-ab138514'>ab138514</a>) at 1/1000 dilution
Lane 1:
Wild-type HCT 116 (Human colorectal carcinoma cell line) whole cell lysate at 20 µg
Lane 2:
PKN2 knockout HCT 116 (Human colorectal carcinoma cell line) whole cell lysate at 20 µg
Lane 2:
Western blot - Human PKN2 knockout HCT116 cell line (<a href='/en-us/products/cell-lines/human-pkn2-knockout-hct116-cell-line-ab266894'>ab266894</a>)
Lane 3:
HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution
Predicted band size: 112 kDa
Observed band size: 125 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human PKN2 knockout HCT116 cell lysate (AB258588)
Allele-2 : 1 bp insertion in exon5
- Sanger seq
Unknown
Sanger Sequencing - Human PKN2 knockout HCT116 cell lysate (AB258588)
Allele-1 : 23 bp deletion in exon5
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Complementary Products
![Western blot - Anti-PKN2 antibody [EPR5490] (AB138514)](https://content.abcam.com/products/images/pkn2-antibody-epr5490-ab138514--western-blot-img6982.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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