PKN2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon5 and 23 bp deletion in exon5.
Images
Key facts
- Cell type
- HCT116
- Species or organism
- Human
- Tissue
- Colon
- Knockout validation
- Sanger Sequencing, Western blot
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon5 and 23 bp deletion in exon5.
Alternative names
Cardiolipin activated protein kinase Pak2, MGC150606, MGC71074, PKN gamma, PKN2_HUMAN, PRK2, PRKCL2, PRO2042, Protein kinase C-like 2, Protein kinase N2, Protein-kinase C-related kinase 2, Serine/threonine-protein kinase N2
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PKN2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon5 and 23 bp deletion in exon5.
Key facts
- Cell type
- HCT116
- Species or organism
- Human
- Tissue
- Colon
- Knockout validation
- Sanger Sequencing, Western blot
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon5 and 23 bp deletion in exon5.
- Disease
- Carcinoma
- Concentration
Properties
- Gene name
- PKN2
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing, Western blot
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 1 US: 1
- Adherent/suspension
- Adherent
- Gender
- Male
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Western blot - Human PKN2 knockout HCT116 cell lysate (ab258588)
Lane 1:Wild-type HCT 116 (Human colorectal carcinoma cell line) whole cell lysate (20 ug)
Lane 2:PKN2 knockout HCT 116 (Human colorectal carcinoma cell line) whole cell lysate (20 ug)
Lane 3:HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (20 ug)Anti-PKN2 antibody [EPR5490] ab138514 was shown to specifically react with PKN2 in wild-type HCT cells. Loss of signal was observed when knockout cell line Human PKN2 knockout HCT116 cell line ab266894 (knockout cell lysate ab258588) was used. Wild-type and PKN2 knockout samples were subjected to SDS-PAGE. Anti-PKN2 antibody [EPR5490] ab138514 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-PKN2 antibody [EPR5490] (Anti-PKN2 antibody [EPR5490] ab138514) at 1/1000 dilution
Lane 1: Wild-type HCT 116 (Human colorectal carcinoma cell line) whole cell lysate at 20 µg
Lane 2: PKN2 knockout HCT 116 (Human colorectal carcinoma cell line) whole cell lysate at 20 µg
Lane 2: Western blot - Human PKN2 knockout HCT116 cell line (Human PKN2 knockout HCT116 cell line ab266894)
Lane 3: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution
Predicted band size: 112 kDa
Observed band size: 125 kDa
Sanger Sequencing - Human PKN2 knockout HCT116 cell lysate (ab258588)
Allele-1: 23 bp deletion in exon5
Sanger Sequencing - Human PKN2 knockout HCT116 cell lysate (ab258588)
Allele-2: 1 bp insertion in exon5
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Product protocols
- Visit the general protocols
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