Human PKN2 knockout HeLa cell lysate
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PKN2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 5.
View Alternative Names
Cardiolipin activated protein kinase Pak2, MGC150606, MGC71074, PKN gamma, PKN2_HUMAN, PRK2, PRKCL2, PRO2042, Protein kinase C-like 2, Protein kinase N2, Protein-kinase C-related kinase 2, Serine/threonine-protein kinase N2
- WB
Unknown
Western blot - Human PKN2 knockout HeLa cell lysate (AB258587)
Lane 1 : Wild-type HeLa cell lysate (20 μg)
Lane 2 : PKN2 knockout HeLa cell lysate (20 μg)
Lane 3 : 293T cell lysate (20 μg)
Lanes 1-3 : Merged signal (red and green). Green - ab138514 observed at 112 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab138514 Anti-PKN2 antibody [EPR5490] was shown to specifically react with PKN2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264691 (knockout cell lysate ab258587) was used. Wild-type and PKN2 knockout samples were subjected to SDS-PAGE. ab138514 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-PKN2 antibody [EPR5490] (<a href='/en-us/products/primary-antibodies/pkn2-antibody-epr5490-ab138514'>ab138514</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
PKN2 knockout HeLa cell lysate at 20 µg
Lane 3:
293T cell lysate at 20 µg
Predicted band size: 112 kDa
Observed band size: 112 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human PKN2 knockout HeLa cell lysate (AB258587)
Homozygous : 1 bp insertion in exon 5
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
![Western blot - Anti-PKN2 antibody [EPR5490] (AB138514)](https://content.abcam.com/products/images/pkn2-antibody-epr5490-ab138514--western-blot-img6982.jpg)
Product promise
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