Human PLA2G12A (Phospholipase A2 XII) knockout HeLa cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
- Sanger seq
Unknown
Sanger Sequencing - Human PLA2G12A (Phospholipase A2 XII) knockout HeLa cell lysate (AB263299)
Homozygous : 10 bp deletion in exon1
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The enzyme belongs to the large group of secreted phospholipases A2 that contribute to diverse biological processes despite its low catalytic activity. It does not form part of a complex but often interacts with other molecules during lipid metabolism. Also it participates in lipid mediator production which impacts inflammation and host defense mechanisms. Its presence in immune cells indicates a potential involvement in immune responses and tissue homeostasis.
Pathways
PLA2G12A is essential in the arachidonic acid metabolism pathway and inflammatory signaling pathways. It connects with other proteins such as secretory phospholipase A2 and cytosolic phospholipase A2. The enzyme's action leads to the release of lipid mediators that modulate inflammatory pathways highlighting its regulatory role in these networks. It influences the balance and interplay between the production and degradation of lipid mediators important for biological signaling.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com