Human PLAA knockout HeLa cell lysate
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PLAA KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Insertion of the selection cassette in exon3.
View Alternative Names
FLJ11281, FLJ12699, Glycerophosphatase, OTTHUMP00000045176, PLA2P, PLAP_HUMAN, Phospholipase A-2-activating protein, Plaa
- WB
Lab
Western blot - Human PLAA knockout HeLa cell lysate (AB258121)
Lane 1 : Wild-type HeLa cell lysate (20 μg)
Lane 2 : PLAA knockout HeLa cell lysate (20 μg)
Lane 3 : MCF7 cell lysate (20 μg)
Lane 4 : Daudi cell lysate (20 μg)
Lanes 1-4 : Merged signal (red and green). Green - ab133589 observed at 87 kDa. Red - loading control ab8245 observed at 37 kDa.
ab133589 Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] was shown to specifically react with PLAP in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265255 (knockout cell lysate ab258121) was used. Wild-type and PLAP knockout samples were subjected to SDS-PAGE. ab133589 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (<a href='/en-us/products/primary-antibodies/phospholipase-a2-activator-protein-plap-antibody-epr5481-ab133589'>ab133589</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
PLAP knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human PLAA knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-plaa-knockout-hela-cell-line-ab265255'>ab265255</a>)
Lane 3:
MCF7 cell lysate at 20 µg
Lane 4:
Daudi cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution
Predicted band size: 87 kDa
Observed band size: 87 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human PLAA knockout HeLa cell lysate (AB258121)
Homozygous : Insertion of the selection cassette in exon3
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This protein actively participates in cellular responses by modulating the production of lipid mediators. PLAP does not work standalone; it forms part of the calcium-dependent phospholipid-binding complex. Through its role in this complex it influences numerous processes such as inflammation and immune response. PLAP is also essential for the optimal function of inflammatory cell membranes by regulating the release of pro-inflammatory eicosanoids.
Pathways
This protein contributes significantly to the arachidonic acid metabolism pathway and eicosanoid biosynthesis pathway. In these pathways PLAP ensures the effectiveness of phospholipase A2 enabling the release of arachidonic acid a precursor for prostaglandins and leukotrienes. These components influence key activities in cellular signaling. Proteins such as cyclooxygenase (COX) and lipoxygenase follow through these pathways affecting cellular responses.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB133589
Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481]
RabMAb|Recombinant|KO Validated
![Western blot - Anti-Phospholipase A2 activator protein (PLAP) antibody [EPR5481] (AB133589)](https://content.abcam.com/products/images/phospholipase-a2-activator-protein-plap-antibody-epr5481-ab133589--western-blot-img71242.jpg)
- 0
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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