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AB258122

Human PLCB3 (Phospholipase C beta 3) knockout HeLa cell lysate

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PLCB3 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 4 bp deletion in exon2 and Insertion of the selection cassette in exon2.

View Alternative Names

1 phosphatidylinositol 4 5 bisphosphate phosphodiesterase beta 3, 1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase beta-3, PLC-beta-3, PLCB3_HUMAN, Phosphoinositide phospholipase C, Phosphoinositide phospholipase C-beta-3, Phospholipase C beta 3 (phosphatidylinositol specific), Phospholipase C-beta-3

4 Images
Western blot - Human PLCB3 (Phospholipase C beta 3) knockout HeLa cell lysate (AB258122)
  • WB

Lab

Western blot - Human PLCB3 (Phospholipase C beta 3) knockout HeLa cell lysate (AB258122)

Lane 1 : Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate, 20 ug
Lane 2 : PLCB3 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate, 20 ug

Lanes 1 - 2 : Merged signal (red and green). Green - ab124735 observed at 150 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37 kDa.

ab124735 was shown to react with Phospholipase C beta 3/PLCB3 in wild-type HeLa cells in Western blot with loss of signal observed in PLCB3 knockout cell line ab266034 (PLCB3 knockout cell lysate ab258122). Wild-type HeLa and PLCB3 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab124735 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-Phospholipase C beta 3/PLCB3 antibody [EPR5951] (<a href='/en-us/products/primary-antibodies/phospholipase-c-beta-3-plcb3-antibody-epr5951-ab124735'>ab124735</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

PLCB3 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

Western blot - Human PLCB3 (Phospholipase C beta 3) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-plcb3-phospholipase-c-beta-3-knockout-hela-cell-line-ab266034'>ab266034</a>)

Predicted band size: 139 kDa

Observed band size: 150 kDa

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Sanger Sequencing - Human PLCB3 (Phospholipase C beta 3) knockout HeLa cell lysate (AB258122)
  • Sanger seq

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Sanger Sequencing - Human PLCB3 (Phospholipase C beta 3) knockout HeLa cell lysate (AB258122)

Allele-1 : 4 bp deletion in exon2

Sanger Sequencing - Human PLCB3 (Phospholipase C beta 3) knockout HeLa cell lysate (AB258122)
  • Sanger seq

Unknown

Sanger Sequencing - Human PLCB3 (Phospholipase C beta 3) knockout HeLa cell lysate (AB258122)

Allele-2 : Insertion of the selection cassette in exon2

Sanger Sequencing - Human PLCB3 (Phospholipase C beta 3) knockout HeLa cell lysate (AB258122)
  • Sanger seq

Unknown

Sanger Sequencing - Human PLCB3 (Phospholipase C beta 3) knockout HeLa cell lysate (AB258122)

Allele-3 : Insertion of the selection cassette in exon2

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 4 bp deletion in exon2 and Insertion of the selection cassette in exon2.

Disease

Adenocarcinoma

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
PLCB3
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phospholipase C beta 3 often called PLCB3 plays an important role in cellular signal transduction. This enzyme exists as part of the phospholipase C family enhances the conversion of phosphatidylinositol 45-bisphosphate (PIP2) into diacylglycerol (DAG) and inositol triphosphate (IP3). These molecules act as secondary messengers and are important to cellular responses. PLCB3 has a molecular mass of approximately 152 kDa. It exhibits significant expression in various tissues including the brain and heart among others where it regulates numerous physiological processes.
Biological function summary

The activation of PLCB3 influences intracellular calcium levels by producing IP3 contributing to calcium signaling pathways. As part of its biological role PLCB3 contributes to a dynamic signaling complex interacting with receptors like G-protein-coupled receptors (GPCRs) and affecting downstream signaling. This enzyme actively participates in regulating cellular processes such as proliferation and differentiation by mediating signal transduction pathways.

Pathways

PLCB3 integrates into the calcium signaling and phosphatidylinositol signaling pathways where it functions through interaction with G alpha q proteins. The activation of PLCB3 leads to the release of calcium ions from the endoplasmic reticulum a process important for many cellular functions. In these pathways PLCB3 shares interactions with other related proteins like protein kinase C (PKC) which further modulates cellular responses.

Dysregulation of PLCB3 activity has links to several pathophysiological conditions including cardiovascular diseases and cancer. Abnormal PLCB3 signaling correlates with irregular calcium homeostasis impacting cardiac functions and promoting oncogenic transformations. Interactions between PLCB3 and proteins such as GPCRs highlight their importance in developing therapeutic targets for treating these diseases.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Complementary Products

Primary Antibodies

AB184743

Anti-Phospholipase C beta 1/PLCB1 + Phospholipase C beta 3/PLCB3 antibody [EPR18714]

RabMAb|Recombinant

Western blot - Anti-Phospholipase C beta 1/PLCB1 + Phospholipase C beta 3/PLCB3 antibody [EPR18714] (AB184743)

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Cell Lines & Lysates

AB266034

Human PLCB3 (Phospholipase C beta 3) knockout HeLa cell line

Advanced Validation

Western blot - Human PLCB3 (Phospholipase C beta 3) knockout HeLa cell line (AB266034)

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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