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AB257590

Human PLCG1 (Phospholipase C gamma 1/PLC-gamma-1) knockout HEK-293T cell lysate

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PLCG1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 267 bp deletion in exon1.

View Alternative Names

1 phosphatidyl D myo inositol 4 5 bisphosphate, 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase gamma-1, Inositoltrisphosphohydrolase, Monophosphatidylinositol phosphodiesterase, NCKAP3, PLC 1, PLC-148, PLC-II, PLC-gamma-1, PLCG1_HUMAN, Phosphatidylinositol phospholipase C, Phosphoinositidase C, Phosphoinositide phospholipase C, Phosphoinositide phospholipase C-gamma-1, Phospholipase C 148, Phospholipase C-II, Phospholipase C-gamma-1

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Western blot - Human PLCG1 (Phospholipase C gamma 1/PLC-gamma-1) knockout HEK-293T cell lysate (AB257590)
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Western blot - Human PLCG1 (Phospholipase C gamma 1/PLC-gamma-1) knockout HEK-293T cell lysate (AB257590)

Lane 1 : Wild-type HEK-293T cell lysate (40ug)

Lane 2 : PLCG1 knockout HEK-293T cell lysate (40ug)

Lanes 1- 2 : Merged signal (red and green). Green - ab109501 observed at 160 kDa. Red - loading control ab8245 observed at 37 kDa.

ab109501 Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] was shown to specifically react with Phospholipase C gamma 1/PLC-gamma-1 in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266530 (knockout cell lysate ab257590) was used. Wild-type and Phospholipase C gamma 1/PLC-gamma-1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab109501 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Phospholipase C gamma 1/PLC-gamma-1 antibody [EPR5358] (<a href='/en-us/products/primary-antibodies/phospholipase-c-gamma-1-plc-gamma-1-antibody-epr5358-ab109501'>ab109501</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 40 µg/mL

Lane 2:

PLCG1 knockout HEK-293T cell lysate at 40 µg/mL

Lane 2:

Western blot - Human PLCG1 (Phospholipase C gamma 1/PLC-gamma-1) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-plcg1-phospholipase-c-gamma-1-plc-gamma-1-knockout-hek-293t-cell-line-ab266530'>ab266530</a>)

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution

Predicted band size: 148 kDa

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Sanger Sequencing - Human PLCG1 (Phospholipase C gamma 1/PLC-gamma-1) knockout HEK-293T cell lysate (AB257590)
  • Sanger seq

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Sanger Sequencing - Human PLCG1 (Phospholipase C gamma 1/PLC-gamma-1) knockout HEK-293T cell lysate (AB257590)

Homozygous : 267 bp deletion in exon1

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 267 bp deletion in exon1.

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab257590-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human PLCG1 knockout HEK293T cell lysate", "number":"AB257590-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HEK293T cell lysate", "number":"AB257590-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Gene name
PLCG1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Phospholipase C gamma 1 (PLC-gamma-1) also known as PLCG1 or PLC-gamma plays an important role in cellular signaling. This enzyme has a molecular mass of approximately 150 kDa. It is found in multiple cell types but is highly expressed in the brain and immune cells. PLC-gamma-1 is involved in the hydrolysis of phosphatidylinositol 45-bisphosphate (PIP2) to generate inositol 145-trisphosphate (IP3) and diacylglycerol (DAG) leading to downstream signaling events that control various cellular processes.
Biological function summary

PLC-gamma-1 is integral in signal transduction and is an important player in the immune response. The protein does not function as part of a large multi-protein complex but interacts transiently with other signaling molecules. It acts downstream of growth factor receptors and immunoreceptors by mediating calcium release and activation of protein kinase C influencing cell proliferation migration and differentiation. Its expression pattern suggests a critical role in the nervous and immune systems affecting learning memory and immune defense.

Pathways

PLC-gamma-1 is an important component in both the phospholipase C and PLC-gamma pathways. These pathways include important interactions with proteins such as the receptor tyrosine kinases and Src family kinases. In these pathways PLC-gamma-1 modulates signals that guide cellular responses to external stimuli affecting processes like growth signaling and T-cell receptor activation which is vital in adaptive immunity.

PLC-gamma-1 has significant connections to cancer and immune deficiencies. Abnormal PLC-gamma-1 activity is observed in certain cancers such as breast cancer where it influences tumor growth and metastasis. Its dysregulation in immune cells leads to disorders like autoimmune diseases by impacting proteins such as LAT and SLP-76 which are important in T-cell signaling. Understanding PLC-gamma-1’s regulatory mechanisms helps in developing targeted therapies for these conditions.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

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