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AB263302

Human PLEKHJ1 (GNRPX) knockout HEK-293T cell lysate

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PLEKHJ1 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 1.
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Sanger Sequencing - Human PLEKHJ1 (GNRPX) knockout HEK-293T cell lysate (AB263302)
  • Sanger seq

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Sanger Sequencing - Human PLEKHJ1 (GNRPX) knockout HEK-293T cell lysate (AB263302)

Homozygous : 1 bp deletion in exon 1

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 1.

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Complementary Products

Cell Lines & Lysates

AB266645

Human PLEKHJ1 (GNRPX) knockout HEK-293T cell line

Sanger Sequencing - Human PLEKHJ1 (GNRPX) knockout HEK-293T cell line (AB266645)

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
PLEKHJ1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Zygosity
Homozygous
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

GNRPX also known as Gonaderexin Protein X plays a significant mechanical role in cellular processes. It has a molecular mass of approximately 45 kDa. This protein shows expression in the gonadal tissues including the testes and ovaries and can be detected in lower levels elsewhere in the body. GNRPX acts as a regulator within the cellular signaling systems pertinent to reproductive functions.
Biological function summary

GNRPX facilitates communication between cellular systems involved in reproductive biology. It acts as a component of a larger protein complex that participates in the regulation of gonadotropin-releasing activities. These processes are essential for normal reproductive function including the regulation of hormone release. GNRPX does not act independently but within a collaboration of proteins that support the reproductive axis.

Pathways

GNRPX is linked with the hypothalamic-pituitary-gonadal (HPG) axis pathway vital for reproductive hormone signaling. Within this axis GNRPX interacts closely with the luteinizing hormone-releasing hormone (LHRH). It maintains important links to ensure the cascade of events that mediate the release of hormones like luteinizing hormone and follicle-stimulating hormone. Disruptions in these interactions can impact hormonal balance and reproductive health.

GNRPX has associations with conditions related to reproductive health such as hypogonadotropic hypogonadism. Altered expression or mutations in GNRPX might correlate with disrupted hormone regulation in this disorder. It shows connections to other proteins involved in the disorder pathway like LHRH highlighting its integral role in maintaining hormonal homeostasis. Understanding its involvement in these disorders could aid the development of targeted therapies.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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