Human PLOD2 (PLOD2/LH2) knockout HEK-293T cell lysate
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PLOD2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon4.
View Alternative Names
2-oxoglutarate 5-dioxygenase 2, LH2, Lysine hydroxylase 2, Lysyl hydroxylase 2, OTTHUMP00000215204, OTTHUMP00000215205, OTTHUMP00000215206, PLOD2_HUMAN, Procollagen lysine 2 oxoglutarate 5 dioxygenase 2, Procollagen lysine, 2 oxoglutarate 5 dioxygenase (lysine hydroxylase) 2, Procollagen-lysine, TLH, Telopeptide lysyl hydroxylase
- Sanger seq
Unknown
Sanger Sequencing - Human PLOD2 (PLOD2/LH2) knockout HEK-293T cell lysate (AB258591)
Homozygous : 1 bp insertion in exon4
- WB
Supplier Data
Western blot - Human PLOD2 (PLOD2/LH2) knockout HEK-293T cell lysate (AB258591)
Blocking and diluting buffer and concentration : 5% NFDM/TBST
Lysates at 20 µg per lane.
The samples were run on a Bis-Tris gel under reducing conditions. Western blot : Anti-PLOD2/LH2 antibody (ab313765) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody 6C5 (ab8245) loading control staining at 1/20000 dilution, shown in red.
In Western blot, ab313765 was shown to bind specifically to PLOD2/LH2.
Target of interest was observed at 95 kDa in wild-type Hela cell lysates (lane 1) with no signal observed at this size in PLOD2 knockout cell line ab266279 (knockout cell lysate ab258591) (lane 2). To generate this image, samples were first run on an SDS-PAGE gel then transferred onto an immobilon-FL PVDF membrane. Membranes were blocked in a fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4°C. Blots were washed in TBS-T, incubated with secondary antibodies Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution for 1 h at room temperature, washed again then imaged.
All lanes:
Western blot - Anti-PLOD2/LH2 antibody [EPR25160-20] (<a href='/en-us/products/primary-antibodies/plod2-lh2-antibody-epr25160-20-ab313765'>ab313765</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
Lane 2:
Western blot - Human PLOD2 (PLOD2/LH2) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-plod2-plod2-lh2-knockout-hek-293t-cell-line-ab266279'>ab266279</a>)
Lane 2:
Western blot - Human PLOD2 (PLOD2/LH2) knockout HEK-293T cell lysate (ab258591) at 20 µg
Lane 3:
HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 4:
A431 (human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg
Secondary
All lanes:
Goat Anti-Rabbit IgG H&L (800CW) and Goat Anti-Mouse IgG H&L (680RD) at 1/20000 dilution
Observed band size: 95 kDa
false
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB313765
Anti-PLOD2/LH2 antibody [EPR25160-20]
BOND RX™ Validated|20ul selling size|RabMAb|KO Validated|Recombinant
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PLOD2/LH2 antibody [EPR25160-20] (AB313765)](https://content.abcam.com/products/images/plod2-lh2-antibody-epr25160-20-ab313765--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img419865.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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