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AB258592

Human PLS3 (T Plastin/PLS3) knockout HEK-293T cell lysate

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PLS3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 5 and 5 bp deletion in exon 5.

View Alternative Names

BMND18, PLST_HUMAN, Plastin 3 (T isoform), Plastin-3, T fimbrin, T-plastin

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Sanger Sequencing - Human PLS3 (T Plastin/PLS3) knockout HEK-293T cell lysate (AB258592)
  • Sanger seq

Unknown

Sanger Sequencing - Human PLS3 (T Plastin/PLS3) knockout HEK-293T cell lysate (AB258592)

Allele-1 : 5 bp deletion in exon 5

Sanger Sequencing - Human PLS3 (T Plastin/PLS3) knockout HEK-293T cell lysate (AB258592)
  • Sanger seq

Unknown

Sanger Sequencing - Human PLS3 (T Plastin/PLS3) knockout HEK-293T cell lysate (AB258592)

Allele-2 : 1 bp insertion in exon 5

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon 5 and 5 bp deletion in exon 5.

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
PLS3
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The T Plastin also known as PLS3 is a protein with a mass of around 70 kDa. This protein belongs to the plastin family and another popular alias is plastine. T Plastin participates in cellular mechanics by bundling actin filaments which is essential for maintaining cell structure and motility. It occurs in various tissues but is highly expressed in the intestines testis and other cells with rapid movement needs like immune cells. Its presence supports functions needing dynamic rearrangement of the cytoskeleton.
Biological function summary

T Plastin plays significant roles in several cellular activities. It stabilizes the actin cytoskeleton which impacts cell division signal transduction and cellular interactions. T Plastin is not part of a multi-protein complex but works closely with actin and other actin-binding proteins to regulate cytoskeletal organization. By supporting these functions T Plastin indirectly influences cell shape and intracellular transport mechanisms.

Pathways

T Plastin integrates into the actin dynamics pathways important for cytoskeletal reorganization. It works alongside proteins such as cofilin and profilin which also regulate actin dynamics. Another important pathway includes wound healing where T Plastin's actin-bundling function assists in cell migration and tissue regeneration. In these pathways its role ensures efficient cellular responses to environmental changes.

Defects in T Plastin have been linked to osteoporosis and some forms of cancers. In osteoporosis improper expression of T Plastin affects bone density regulation partly due to its influence on osteoclast function. Cancers such as colorectal cancer exhibit altered T Plastin levels which may impact metastasis due to changes in cell motility. In these contexts T Plastin's interaction with other proteins like integrins highlights its importance in controlling cell adhesion and migration associated with disease progress.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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