Human PMS2 knockout HeLa cell lysate
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PMS2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 16 bp deletion in exon2 and 2 bp deletion in exon2 and Insertion of the selection cassette in exon2.
View Alternative Names
DNA mismatch repair gene homologue, DNA mismatch repair protein PMS2, HNPCC4, H_DJ0042M02.9, MLH4, Mismatch repair endonuclease PMS2, Mismatch repair gene PMSL2, PMS1 homolog 2 mismatch repair system, PMS1 protein homolog 2, PMS2 postmeiotic segregation increased 2, PMS2 postmeiotic segregation increased 2 (S. cerevisiae), PMS2CL, PMS2_HUMAN, PMSL2, Postmeiotic segregation increased, S. cerevisiae, 2
- Sanger seq
Lab
Sanger Sequencing - Human PMS2 knockout HeLa cell lysate (AB257142)
Allele-3 : Insertion of the selection cassette in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human PMS2 knockout HeLa cell lysate (AB257142)
Allele-1 : 16 bp deletion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human PMS2 knockout HeLa cell lysate (AB257142)
Allele-2 : 2 bp deletion in exon2
- WB
Lab
Western blot - Human PMS2 knockout HeLa cell lysate (AB257142)
Lanes 1- 2 : Merged signal (red and green). Green - ab110638 observed at 120 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.
ab110638 was shown to react with PMS2 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab261776 (knockout cell lysate ab257142) was used. Wild-type HeLa and PMS2 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab110638 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-PMS2 antibody [EPR3947] (<a href='/en-us/products/primary-antibodies/pms2-antibody-epr3947-ab110638'>ab110638</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human PMS2 knockout HeLa cell lysate (ab257142) at 20 µg
Secondary
Lanes 1 - 2:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution
Lanes 1 - 2:
Western blot - Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>) at 1/20000 dilution
Predicted band size: 96 kDa
Observed band size: 120 kDa,37 kDa
false
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PMS2 operates as part of the DNA mismatch repair (MMR) complex. It collaborates with other MutL homologs including MLH1 to form a heterodimer which is essential for repairing DNA replication errors. It safeguards genomic integrity and prevents mutations from accumulating in dividing cells serving important functions in cellular viability and genetic stability.
Pathways
PMS2 is involved in the DNA damage response and cell cycle regulation. The protein plays a vital role in the mismatch repair (MMR) pathway. PMS2 partners primarily with MLH1 within this pathway and both proteins work in conjunction to recognize and initiate repair on erroneous DNA sequences that emerge during replication preventing illegitimate recombination and chromosomal rearrangements.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB110638
BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size|Trial Size|Trial Size
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PMS2 antibody [EPR3947] (AB110638)](https://content.abcam.com/products/images/pms2-antibody-epr3947-ab110638--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img186217.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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