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PMS2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 16 bp deletion in exon2 and 2 bp deletion in exon2 and Insertion of the selection cassette in exon2.

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Images

Western blot - Human PMS2 knockout HeLa cell lysate (AB257142), expandable thumbnail
  • Sanger Sequencing - Human PMS2 knockout HeLa cell lysate (AB257142), expandable thumbnail
  • Sanger Sequencing - Human PMS2 knockout HeLa cell lysate (AB257142), expandable thumbnail
  • Sanger Sequencing - Human PMS2 knockout HeLa cell lysate (AB257142), expandable thumbnail

Key facts

Cell type
HeLa
Species or organism
Human
Tissue
Cervix
Knockout validation
Sanger Sequencing, Western blot
Mutation description
Knockout achieved by using CRISPR/Cas9, 16 bp deletion in exon2 and 2 bp deletion in exon2 and Insertion of the selection cassette in exon2.

Alternative names

DNA mismatch repair gene homologue, DNA mismatch repair protein PMS2, HNPCC4, H_DJ0042M02.9, MLH4, Mismatch repair endonuclease PMS2, Mismatch repair gene PMSL2, PMS1 homolog 2 mismatch repair system, PMS1 protein homolog 2, PMS2 postmeiotic segregation increased 2, PMS2 postmeiotic segregation increased 2 (S. cerevisiae), PMS2CL, PMS2_HUMAN, PMSL2, Postmeiotic segregation increased, S. cerevisiae, 2

What's included?

1 Kit
Components
Human PMS2 knockout HeLa cell lysate
1 x 100 µg
Human wild-type HeLa cell lysate
1 x 100 µg

Recommended products

PMS2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 16 bp deletion in exon2 and 2 bp deletion in exon2 and Insertion of the selection cassette in exon2.

Key facts

Cell type
HeLa
Mutation description
Knockout achieved by using CRISPR/Cas9, 16 bp deletion in exon2 and 2 bp deletion in exon2 and Insertion of the selection cassette in exon2.
Disease
Adenocarcinoma
Concentration
Loading...

Properties

Gene name
PMS2
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot

Quality control

STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female

Storage

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C

Notes


Knockout cell lysate achieved by CRISPR/Cas9.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PMS2 also known as Postmeiotic Segregation Increased 2 or PMS2 MutL homolog is a protein that participates in DNA mismatch repair. It weighs approximately 96 kDa and often identifies as a member of the MutL protein family. PMS2 is ubiquitously expressed in the body with higher abundances in tissues that undergo rapid proliferation or possess a high mitotic index.

Biological function summary

PMS2 operates as part of the DNA mismatch repair (MMR) complex. It collaborates with other MutL homologs including MLH1 to form a heterodimer which is essential for repairing DNA replication errors. It safeguards genomic integrity and prevents mutations from accumulating in dividing cells serving important functions in cellular viability and genetic stability.

Pathways

PMS2 is involved in the DNA damage response and cell cycle regulation. The protein plays a vital role in the mismatch repair (MMR) pathway. PMS2 partners primarily with MLH1 within this pathway and both proteins work in conjunction to recognize and initiate repair on erroneous DNA sequences that emerge during replication preventing illegitimate recombination and chromosomal rearrangements.

Associated diseases and disorders

PMS2 mutations occur frequently in Lynch syndrome an inherited cancer predisposition disorder and Turcot syndrome a condition associated with colorectal cancer and brain tumors. MLH1 frequently associates with PMS2 in these disorders as defects in either protein can impair mismatch repair leading to an increased risk of cancer.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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