Human PNKD knockout HeLa cell lysate
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PNKD KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 3 bp deletion in exon 1 and 4 bp deletion in exon 1.
View Alternative Names
2210013N15Rik, 2810403H05Rik, AI854243, BRAIN PROTEIN 17, BRP17, DYT8, FKSG19, FPD1, KIAA1184, KIPP1184, MNCb-5687, MR-1, Myofibrillogenesis regulator 1, PDC, PKND1, PNKD_HUMAN, Paroxysmal nonkinesiogenic dyskinesia protein, Probable hydrolase PNKD, TAHCCP2, Trans-activated by hepatitis C virus core protein 2
- Sanger seq
Unknown
Sanger Sequencing - Human PNKD knockout HeLa cell lysate (AB263305)
Allele-1 : 4 bp deletion in exon 1
- Sanger seq
Unknown
Sanger Sequencing - Human PNKD knockout HeLa cell lysate (AB263305)
Allele-2 : 3 bp deletion in exon 1
- Sanger seq
Unknown
Sanger Sequencing - Human PNKD knockout HeLa cell lysate (AB263305)
Allele-3 :
- Sanger seq
Unknown
Sanger Sequencing - Human PNKD knockout HeLa cell lysate (AB263305)
Allele-4 :
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PNKD is involved in muscle contraction and neurological signaling. It is a part of a larger complex that affects calcium ion binding and muscle contraction. By aiding in the assembly of sarcomeric structures PNKD ensures the stability and proper functioning of muscle fibers. In the nervous system it appears to participate in modulating synaptic activity implying a role in neural communication and plasticity.
Pathways
PNKD participates in intracellular signaling associated with muscle contraction and synaptic function. In the calcium signaling pathway it interacts with proteins such as actin and tropomyosin to facilitate muscle function. Furthermore PNKD associates with pathways affecting neurotransmitter release at synapses implicating proteins like synapsin and SNAP-25 which are important for synaptic vesicle docking and release.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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