Human POGK knockout HeLa cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human POGK knockout HeLa cell lysate (AB263306)
Allele-2 : 1 bp deletion in exon4
- Sanger seq
Unknown
Sanger Sequencing - Human POGK knockout HeLa cell lysate (AB263306)
Allele-1 : 2 bp deletion in exon4
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
POGK participates in regulating gene expression through its transcriptional repression activity. The protein forms part of a larger repressive complex known as the KRAB-ZFP/KAP1 network. This complex engages with histone methyltransferases to modify histones which results in transcriptional silencing of target genes. POGK thereby contributes to maintaining chromatin structure and regulating genomic stability.
Pathways
POGK engages in epigenetic regulation pathways influencing gene silencing and chromatin remodeling. It works closely with members of the KRAB-ZFP family and KAP1 key regulators in these pathways. POGK's activity impacts the silencing of endogenous retroviruses important for normal developmental processes and cellular differentiation.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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