Human POLDIP3 (p46) knockout HeLa cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
POLDIP3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon 1 and 1 bp insertion in exon 1.
View Alternative Names
46 kDa DNA polymerase delta interaction protein, DNA polymerase delta interacting protein 3, KIAA1649, PDIP3_HUMAN, PDIP46, POLDIP3, Polymerase (DNA-directed), delta interacting protein 3, Polymerase delta interacting protein 46, Polymerase delta-interacting protein 3, RNA-binding protein P46, S6K1 Aly/REF-like target, SKAR
- Sanger seq
Unknown
Sanger Sequencing - Human POLDIP3 (p46) knockout HeLa cell lysate (AB259047)
Allele-2 : 1 bp insertion in exon 1
- Sanger seq
Unknown
Sanger Sequencing - Human POLDIP3 (p46) knockout HeLa cell lysate (AB259047)
Allele-1 : 14 bp deletion in exon 1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Heat shock protein 46 is integral in cellular protection during stress conditions. It participates in forming complexes with other heat shock proteins enhancing its function in assisting proteins during synthesis and repair. This protein acts in maintaining the stability and function of other proteins preserving cellular health under stress. Its involvement in cellular processes signifies its importance in both cellular functioning and stress response mechanisms.
Pathways
P46 plays a critical role in the heat shock response and protein quality control pathways. It interacts with proteins such as HSP70 and HSP90 which participate in similar pathways ensuring proper folding and stability of newly synthesized proteins. These pathways protect cells from damage during stress connecting p46 to broader physiological responses. Its presence in these pathways emphasizes its significance in cellular recovery and adaptation processes.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com