Human POLL (DNA Polymerase lambda) knockout HeLa cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
POLL KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2 and Insertion of the selection cassette in exon2.
View Alternative Names
BETA N, DNA directed DNA polymerase lambda, DNA polymerase beta-2, DNA polymerase kappa, DNA polymerase kappa DNA polymerase beta N, DNA polymerase lambda, DNA polymerase lamda2, DPOLL_HUMAN, EC 2.7.7.7,EC 4.2.99., FLJ46002, OTTHUMP00000020321, OTTHUMP00000020323, OTTHUMP00000059179, POL KAPPA, POLL, Pol Lambda, Pol beta2, Polymerase DNA directed lambda
- WB
Unknown
Western blot - Human POLL (DNA Polymerase lambda) knockout HeLa cell lysate (AB258595)
Lane 1 : Wild-type HeLa cell lysate (20 μg)
Lane 2 : POLL knockout HeLa cell lysate (20 μg)
Lane 3 A549 cell lysate (20 μg)
Lanes 1-3 : Merged signal (red and green). Green - ab172974 observed at 75 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab172974 Anti-DNA Polymerase lambda/Polk antibody [EPR7519(2)] was shown to specifically react with DNA Polymerase lambda/Polk in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264754 (knockout cell lysate ab258595) was used. Wild-type and DNA Polymerase lambda/Polk knockout samples were subjected to SDS-PAGE. ab172974 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-DNA Polymerase lambda antibody [EPR7519(2)] (<a href='/en-us/products/primary-antibodies/dna-polymerase-lambda-antibody-epr75192-ab172974'>ab172974</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
POLL knockout HeLa cell lysate at 20 µg
Lane 3:
A549 cell lysate at 20 µg
Predicted band size: 63 kDa
Observed band size: 75 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human POLL (DNA Polymerase lambda) knockout HeLa cell lysate (AB258595)
Allele-3 : Insertion of the selection cassette in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human POLL (DNA Polymerase lambda) knockout HeLa cell lysate (AB258595)
Allele-2 : Insertion of the selection cassette in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human POLL (DNA Polymerase lambda) knockout HeLa cell lysate (AB258595)
Allele-1 : 1 bp deletion in exon2
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
DNA polymerase lambda acts as a gap-filling enzyme during DNA repair processes especially in base excision repair and non-homologous end joining. This polymerase does not typically function within a larger complex but requires accessory factors to perform its repair functions. It maintains genomic stability by providing an accurate extension of DNA strands during repair ensuring the correct rejoining of DNA. Furthermore it also exhibits a lyase activity that can remove 5'-deoxyribose phosphate residues.
Pathways
DNA polymerase lambda is actively involved in the base excision repair and non-homologous end joining pathways. These pathways are important for fixing DNA single-strand breaks and double-strand breaks respectively. Pol λ works alongside proteins like XRCC1 in the short-patch base excision repair pathway and interacts with KU70/80 in non-homologous end joining reinforcing its multifaceted role in maintaining DNA integrity under varying genotoxic stress conditions.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Cell Lines & Lysates
AB264754
Human POLL (DNA Polymerase lambda) knockout HeLa cell line
Advanced Validation
- 0
- 0
Primary Antibodies
AB172974
Anti-DNA Polymerase lambda antibody [EPR7519(2)]
RabMAb|Recombinant|KO Validated
![Western blot - Anti-DNA Polymerase lambda antibody [EPR7519(2)] (AB172974)](https://content.abcam.com/products/images/dna-polymerase-lambda-antibody-epr75192-ab172974--western-blot-img105148.jpg)
- 0
- 0
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com