Human PON2 knockout HeLa cell lysate
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PON2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon4.
View Alternative Names
A-esterase 2, Aromatic esterase 2, PON2_HUMAN, Paraoxonase 2, Paraoxonase nirs, Serum aryldialkylphosphatase 2, Serum paraoxonase/arylesterase 2
- WB
Unknown
Western blot - Human PON2 knockout HeLa cell lysate (AB258128)
Lane 1 : Wild-type HeLa cell lysate (20 μg)
Lane 2 : PICALM knockout HeLa cell lysate (20 μg)
Lane 3 : PON2 cell lysate (20 μg)
Lanes 1-3 : Merged signal (red and green). Green - ab40969 observed at 39,41 kDa. Red - loading control ab7291 observed at 50 kDa.
ab40969 Anti-PON2 antibody was shown to specifically react with PON2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265927 (knockout cell lysate ab258128) was used. Wild-type and PON2 knockout samples were subjected to SDS-PAGE. ab40969 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-PON2 antibody (<a href='/en-us/products/primary-antibodies/pon2-antibody-ab40969'>ab40969</a>) at 1/500 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
PON2 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human PON2 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-pon2-knockout-hela-cell-line-ab265927'>ab265927</a>)
Lane 3:
HepG2 cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa,41 kDa
false
- WB
Lab
Western blot - Human PON2 knockout HeLa cell lysate (AB258128)
Lane 1 : Wild-type HeLa cell lysate (20 μg)
Lane 2 : PICALM knockout HeLa cell lysate (20 μg)
Lane 3 : PON2 cell lysate (20 μg)
Lanes 1-3 : Merged signal (red and green). Green - ab183710 observed at 39,41 kDa. Red - loading control ab7291 observed at 50 kDa.
ab183710 Anti-PON2 antibody [EPR15295-82] was shown to specifically react with PON2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265927 (knockout cell lysate ab258128) was used. Wild-type and PON2 knockout samples were subjected to SDS-PAGE. ab183710 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-PON2 antibody [EPR15295-82] (<a href='/en-us/products/primary-antibodies/pon2-antibody-epr15295-82-ab183710'>ab183710</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
PON2 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human PON2 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-pon2-knockout-hela-cell-line-ab265927'>ab265927</a>)
Lane 3:
HepG2 cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution
Predicted band size: 39 kDa
Observed band size: 39 kDa,41 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human PON2 knockout HeLa cell lysate (AB258128)
Homozygous : 1 bp deletion in exon4
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
![Western blot - Anti-PON2 antibody [EPR15295-82] (AB183710)](https://content.abcam.com/products/images/pon2-antibody-epr15295-82-ab183710--western-blot-img103768.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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