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AB257600

Human PPID (Cyclophilin 40) knockout HeLa cell lysate

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PPID KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1.

View Alternative Names

40 kDa peptidyl prolyl cis trans isomerase D, 40 kDa peptidyl-prolyl cis-trans isomerase, CYP-40, CyP-D, Cyclophilin D, Cyclophilin-40, Cyclophilin-related protein, MGC33096, PPID_HUMAN, PPIase, PPIase D, Peptidyl Prolyl Isomerase D, Peptidyl-prolyl cis-trans isomerase D, Rotamase, Rotamase D

2 Images
Western blot - Human PPID (Cyclophilin 40) knockout HeLa cell lysate (AB257600)
  • WB

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Western blot - Human PPID (Cyclophilin 40) knockout HeLa cell lysate (AB257600)

Lane 1 : Wild-type HeLa cell lysate (20 ug)
Lane 2 : PPID knockout HeLa cell lysate (20 ug)
Lane 3 : K-562 cell lysate (20 ug)

ab181983 was shown to specifically react with Cyclophilin 40 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265788 (knockout cell lysate ab257600) was used. Wild-type and Cyclophilin 40 knockout samples were subjected to SDS-PAGE. ab181983 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Cyclophilin 40 antibody [EPR14845(B)] (<a href='/en-us/products/primary-antibodies/cyclophilin-40-antibody-epr14845b-ab181983'>ab181983</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

PPID knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human PPID (Cyclophilin 40) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-ppid-cyclophilin-40-knockout-hela-cell-line-ab265788'>ab265788</a>)

Lane 3:

K-562 cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 41 kDa

Observed band size: 47 kDa

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Sanger Sequencing - Human PPID (Cyclophilin 40) knockout HeLa cell lysate (AB257600)
  • Sanger seq

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Sanger Sequencing - Human PPID (Cyclophilin 40) knockout HeLa cell lysate (AB257600)

Homozygous : 1 bp insertion in exon1

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1.

Disease

Adenocarcinoma

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
PPID
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Primary Antibodies

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Product promise

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