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AB257039

Human PPIF (Cyclophilin F) knockout HEK-293T cell lysate

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PPIF KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.

View Alternative Names

CYP3, CyP-D, CyP-M, Cyclophilin 3, Cyclophilin D, Cyclophilin F, Mitochondrial cyclophilin, PPIF_HUMAN, PPIase, PPIase F, Peptidyl prolyl cis trans isomerase, mitochondral, Peptidyl-prolyl cis-trans isomerase F, Peptidyl-prolyl cis-trans isomerase F, mitochondrial, Peptidylprolyl isomerase F, Peptidylprolyl isomerase F (cyclophilin F), Rotamase, Rotamase F, hCyP3, mitochondrial

3 Images
Western blot - Human PPIF (Cyclophilin F) knockout HEK-293T cell lysate (AB257039)
  • WB

Lab

Western blot - Human PPIF (Cyclophilin F) knockout HEK-293T cell lysate (AB257039)

Lane 1 : Wild-type HEK-293T cell lysate (20µg)

Lane 2 : PPIF knockout HEK-293T cell lysate (20µg)

Lanes 1- 2 : Merged signal (red and green). Green - ab231155 observed at 23 kDa. Red - loading control ab8245 observed at 37 kDa.

ab231155 Anti-Cyclophilin F antibody [EPR11311-121] was shown to specifically react with Cyclophilin F in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266077 (knockout cell lysate ab257039) was used. Wild-type and Cyclophilin F knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab231155 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Cyclophilin F antibody [EPR11311-121] (<a href='/en-us/products/primary-antibodies/cyclophilin-f-antibody-epr11311-121-ab231155'>ab231155</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

PPIF knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human PPIF (Cyclophilin F) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-ppif-cyclophilin-f-knockout-hek-293t-cell-line-ab266077'>ab266077</a>)

Predicted band size: 22 kDa

Observed band size: 23 kDa

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Sanger Sequencing - Human PPIF (Cyclophilin F) knockout HEK-293T cell lysate (AB257039)
  • Sanger seq

Unknown

Sanger Sequencing - Human PPIF (Cyclophilin F) knockout HEK-293T cell lysate (AB257039)

Homozygous : 1 bp insertion in exon 1

Western blot - Human PPIF (Cyclophilin F) knockout HEK-293T cell lysate (AB257039)
  • WB

Lab

Western blot - Human PPIF (Cyclophilin F) knockout HEK-293T cell lysate (AB257039)

Lane 1 : Wild-type HEK-293T cell lysate (20µg)

Lane 2 : PPIF knockout HEK-293T cell lysate (20µg)

Lanes 1- 2 : Merged signal (red and green). Green - ab110324 observed at 23 kDa. Red - loading control ab181602 observed at 37 kDa.

ab110324 Anti-Cyclophilin F antibody [E11AE12BD4] was shown to specifically react with Cyclophilin F in wild-type HEK-293T cells in western blot. Loss of signal was observed when knockout cell line ab266077 (knockout cell lysate ab257039) was used. Wild-type and Cyclophilin F knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab110324 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 10000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Cyclophilin F antibody [E11AE12BD4] (<a href='/en-us/products/primary-antibodies/cyclophilin-f-antibody-e11ae12bd4-ab110324'>ab110324</a>) at 1/10000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human PPIF (Cyclophilin F) knockout HEK-293T cell lysate (ab257039) at 20 µg

Predicted band size: 22 kDa

Observed band size: 37 kDa,23 kDa

false

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
PPIF
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Zygosity
Homozygous
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Complementary Products

Primary Antibodies

AB110324

Anti-Cyclophilin F antibody [E11AE12BD4]

KO Validated

Flow Cytometry - Anti-Cyclophilin F antibody [E11AE12BD4] (AB110324)

  • 4
  • 4
Primary Antibodies

AB181983

Anti-Cyclophilin 40 antibody [EPR14845(B)]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin 40 antibody [EPR14845(B)] (AB181983)

  • 0
  • 0
Cell Lines & Lysates

AB266077

Human PPIF (Cyclophilin F) knockout HEK-293T cell line

Advanced Validation

Western blot - Human PPIF (Cyclophilin F) knockout HEK-293T cell line (AB266077)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20

Product promise

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For full details, please see our Terms & Conditions

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