Human PPM1B knockout HEK-293T cell lysate
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PPM1B KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp deletion in exon 3.
View Alternative Names
MGC21657, OTTHUMP00000158953, OTTHUMP00000158954, OTTHUMP00000158955, OTTHUMP00000202394, OTTHUMP00000202395, OTTHUMP00000202399, PP2C beta X, PP2C-beta, PP2CB, PPC2BETAX, PPM1B_HUMAN, Protein phosphatase 1B, Protein phosphatase 1B magnesium dependent beta isoform, Protein phosphatase 2C beta isoform, Protein phosphatase 2C isoform beta, Protein phosphatase 2C like protein, Protein phosphatase, Mg2+/Mn2+ dependent, 1B, protein phosphatase 1B (formerly 2C) magnesium-dependent beta isoform
- Sanger seq
Unknown
Sanger Sequencing - Human PPM1B knockout HEK-293T cell lysate (AB258597)
Homozygous : 2 bp deletion in exon 3
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
PPM1B influences diverse cellular processes such as cell cycle regulation and stress response. It is not part of a large complex but interacts with specific substrates to exert its effects. PPM1B can impact apoptosis and cell differentiation through its action on certain substrates. Its regulatory function is important for maintaining cellular homeostasis and adaptation to environmental changes.
Pathways
Multiple signaling cascades demonstrate the involvement of PPM1B. It plays a significant role in the regulation of the p38 MAPK pathway which is important for stress response and inflammatory processes. In this pathway PPM1B interacts with MAPKAPK2 modulating its activity. PPM1B also participates in the TGF-beta signaling pathway where it can affect the phosphorylation status of SMADs. These interactions emphasize PPM1B's regulatory importance in response to extracellular signals.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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