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AB259056

Human PPM1H knockout HeLa cell lysate

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PPM1H KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon1.
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Sanger Sequencing - Human PPM1H knockout HeLa cell lysate (AB259056)
  • Sanger seq

Unknown

Sanger Sequencing - Human PPM1H knockout HeLa cell lysate (AB259056)

Homozygous : 2 bp deletion in exon1

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon1.

Disease

Adenocarcinoma

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Complementary Products

Primary Antibodies

AB303536

Anti-PPM1H antibody [EPR26028-53]

20ul selling size|RabMAb|Recombinant|KO Validated|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PPM1H antibody [EPR26028-53] (AB303536)

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Cell Lines & Lysates

AB265564

Human PPM1H knockout HeLa cell line

Sanger Sequencing - Human PPM1H knockout HeLa cell line (AB265564)

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
PPM1H
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein phosphatase Mg2+/Mn2+ dependent 1H (PPM1H) is an enzyme that regulates cellular functions by removing phosphate groups from specific proteins. Known also as protein phosphatase 1H it has a molecular mass of approximately 55 kDa. It is expressed mainly in the brain but also in other tissues such as heart and kidney. PPM1H plays a role in intracellular signal transduction by dephosphorylating target proteins involved in cellular processes.
Biological function summary

PPM1H modulates cellular signaling pathways impacting various physiological processes. It acts independently and does not form part of any known larger protein complex. Within cells PPM1H may interact with substrates involved in important cellular functions such as growth differentiation and apoptosis. Researchers propose that it acts as a regulatory phosphatase influencing cell cycle and stress response at a cellular level.

Pathways

PPM1H is involved in signaling pathways like the TGF-beta and MAPK pathways. In these pathways it interacts with proteins such as SMAD2 and MAPK modulating their activity through dephosphorylation. The TGF-beta pathway plays an important role in regulating cell growth and differentiation while the MAPK pathway is essential for transmitting extracellular signals to cellular responses including gene expression.

PPM1H has been implicated in nervous system disorders like Parkinson's disease and Alzheimer's disease. Studies suggest that altered PPM1H function can disrupt signaling pathways leading to neuronal degeneration. In Parkinson's disease PPM1H's connection to LRRK2 a protein kinase mutated in some hereditary cases shows potential implications in disease progression due to regulatory interactions.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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