PPP1R13L KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon4 and 2 bp deletion in exon4.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon4 and 2 bp deletion in exon4.
Alternative names
Inhibitor of ASPP protein, NFkB interacting protein 1, NKIP1, PPP1R13B-like protein, PPP1R13L, Protein iASPP, RAI, RelA-associated inhibitor, inhibitor of apoptosis stimulating protein of p53, protein phosphatase 1 regulatory (inhibitor) subunit 13 like
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PPP1R13L KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon4 and 2 bp deletion in exon4.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon4 and 2 bp deletion in exon4.
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- PPP1R13L
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
IASPP also known as inhibitor of apoptosis-stimulating protein of p53 is a regulatory protein with a mass of approximately 96 kDa. It plays an important role in modulating apoptosis functioning as a negative regulator of p53 a well-known tumor suppressor protein. iASPP binds directly to p53 preventing its ability to transactivate target genes associated with cell cycle arrest and apoptosis. This protein is widely expressed in various tissues suggesting its importance in cellular homeostasis and response to stress.
- Biological function summary
IASPP negatively affects p53-mediated apoptosis and cellular senescence. It is part of a complex with PP1 acting as a regulatory subunit that influences several cellular processes by modulating dephosphorylation activities. iASPP's interaction with PP1 further impacts gene expression by affecting the phosphorylation status of various transcription factors. This regulatory mechanism suggests iASPP plays a role in controlling cell growth and death essential processes for maintaining normal cellular function.
- Pathways
IASPP modulates the p53 pathway an essential mechanism for regulating cell cycle and apoptosis. Within this pathway it associates with proteins like ASPP1 ASPP2 and other family members which also influence p53 activity. iASPP has a role in the NF-kB pathway where it influences inflammation and immune responses. Through these pathways iASPP coordinates signals that determine cellular fate connecting it to fundamental processes in cell biology.
- Associated diseases and disorders
IASPP alterations associate with cancer development and progression. Its overexpression often correlates with enhanced tumor growth due to impaired p53 activity. Studies link iASPP to diseases like acute myeloid leukemia (AML) and prostate cancer. Through its interaction with p53 and proteins such as ASPP1 and ASPP2 iASPP provides a potential therapeutic target where modulating its activity might restore proper cellular responses to stress counteracting tumor development.
Product promise
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2 product images
Sanger Sequencing - Human PPP1R13L (iASPP) knockout HeLa cell lysate (ab258598)
Allele-2: 1 bp insertion in exon4
Sanger Sequencing - Human PPP1R13L (iASPP) knockout HeLa cell lysate (ab258598)
Allele-1: 2 bp deletion in exon4
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Product protocols
- Visit the general protocols
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