Human PPP1R13L (iASPP) knockout HeLa cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
PPP1R13L KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon4 and 2 bp deletion in exon4.
View Alternative Names
Inhibitor of ASPP protein, NFkB interacting protein 1, NKIP1, PPP1R13B-like protein, PPP1R13L, Protein iASPP, RAI, RelA-associated inhibitor, inhibitor of apoptosis stimulating protein of p53, protein phosphatase 1 regulatory (inhibitor) subunit 13 like
- Sanger seq
Unknown
Sanger Sequencing - Human PPP1R13L (iASPP) knockout HeLa cell lysate (AB258598)
Allele-1 : 2 bp deletion in exon4
- Sanger seq
Unknown
Sanger Sequencing - Human PPP1R13L (iASPP) knockout HeLa cell lysate (AB258598)
Allele-2 : 1 bp insertion in exon4
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
IASPP negatively affects p53-mediated apoptosis and cellular senescence. It is part of a complex with PP1 acting as a regulatory subunit that influences several cellular processes by modulating dephosphorylation activities. iASPP's interaction with PP1 further impacts gene expression by affecting the phosphorylation status of various transcription factors. This regulatory mechanism suggests iASPP plays a role in controlling cell growth and death essential processes for maintaining normal cellular function.
Pathways
IASPP modulates the p53 pathway an essential mechanism for regulating cell cycle and apoptosis. Within this pathway it associates with proteins like ASPP1 ASPP2 and other family members which also influence p53 activity. iASPP has a role in the NF-kB pathway where it influences inflammation and immune responses. Through these pathways iASPP coordinates signals that determine cellular fate connecting it to fundamental processes in cell biology.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com