Human PPP1R15B knockout HeLa cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human PPP1R15B knockout HeLa cell lysate (AB263309)
Homozygous : 1 bp insertion in exon 1
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The regulatory action of PPP1R15B on eIF2α impacts protein synthesis by reversing the phosphorylation which occurs in response to cellular stress. It functions as part of a multi-protein complex that includes PP1 and possibly other proteins involved in cellular stress responses. As a result it helps in the recovery of protein translation during periods of endoplasmic reticulum stress contributing to cell survival pathways under transient stress conditions.
Pathways
PPP1R15B plays a significant role in the integrated stress response pathway by modulating protein synthesis. It participates in the unfolded protein response (UPR) which is important when cells encounter endoplasmic reticulum stress. Through its interaction with eIF2α and other associated components like PP1 PPP1R15B facilitates cellular adaptation to changing environmental stressors.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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