Human PPP1R16A knockout HEK-293T cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human PPP1R16A knockout HEK-293T cell lysate (AB258600)
Allele-1 : 10 bp deletion in exon 1
- Sanger seq
Unknown
Sanger Sequencing - Human PPP1R16A knockout HEK-293T cell lysate (AB258600)
Allele-2 : 1 bp deletion in exon 1
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
This protein plays a significant role in the regulation of myosin phosphatase activity influencing muscle function. As part of a multi-subunit complex PPP1R16A interacts with PP1 to dephosphorylate myosin light chains which is essential for muscle relaxation. This process ensures proper muscle contraction and relaxation cycles in response to cellular signals.
Pathways
Protein phosphatase activity plays key roles in both the smooth muscle contraction and Rho GTPase signaling pathways. PPP1R16A by regulating myosin light chain phosphorylation status interacts with proteins like Rho-associated coiled-coil containing protein kinase (ROCK) within these pathways. This involvement positions PPP1R16A as a vital player in pathways that govern cell motility and muscle contraction.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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